HS3ST4 Protein (AA 1-456) (His tag)
custom-made
HS3ST4
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all HS3ST4 Proteins
- HS3ST4 (Heparan Sulfate (Glucosamine) 3-O-Sulfotransferase 4 (HS3ST4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-456
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HS3ST4 protein is labelled with His tag.
- Purpose
- Custom-made recombinant HS3ST4 Protein expressed in mammalian cells.
- Sequence
- MARWPAPPPP PPPPPPLAAP PPPGASAKGP PARKLLFMCT LSLSVTYLCY SLLGGSGSLQ FPLALQESPG AAAEPPPSPP PPSLLPTPVR LGAPSQPPAP PPLDNASHGE PPEPPEQPAA PGTDGWGLPS GGGGAQDAWL RTPLAPSEMI TAQSALPERE AQESSTTDED LAGRRAANGS SERGGAVSTP DYGEKKLPQA LIIGVKKGGT RALLEAIRVH PDVRAVGVEP HFFDRNYEKG LEWYRNVMPK TLDGQITMEK TPSYFVTNEA PKRIHSMAKD IKLIVVVRNP VTRAISDYTQ TLSKKPEIPT FEVLAFKNRT LGLIDASWSA IRIGIYALHL ENWLQYFPLS QILFVSGERL IVDPAGEMAK VQDFLGLKRV VTEKHFYFNK TKGFPCLKKP EDSSAPRCLG KSKGRTHPRI DPDVIHRLRK FYKPFNLMFY QMTGQDFQWE QEEGDK Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- HS3ST4 (Heparan Sulfate (Glucosamine) 3-O-Sulfotransferase 4 (HS3ST4))
- Alternative Name
- HS3ST4 (HS3ST4 Products)
- Background
- Heparan sulfate glucosamine 3-O-sulfotransferase 4 (EC 2.8.2.23) (Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4) (3-OST-4) (Heparan sulfate 3-O-sulfotransferase 4) (h3-OST-4),FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate (By similarity). {ECO:0000250}.
- Molecular Weight
- 49.8 kDa
- UniProt
- Q9Y661
- AlphaFold
- Q9Y661
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