METTL13 Protein (AA 1-699) (His tag)
custom-made
METTL13
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all METTL13 products
- METTL13 (Methyltransferase Like 13 (METTL13))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-699
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This METTL13 protein is labelled with His tag.
- Purpose
- Custom-made recombinant METTL13 Protein expressed in mammalian cells.
- Sequence
- MNLLPKSSRE FGSVDYWEKF FQQRGKKAFE WYGTYLELCG VLHKYIKPRE KVLVIGCGNS ELSEQLYDVG YRDIVNIDIS EVVIKQMKEC NATRRPQMSF LKMDMTQMEF PDASFQVVLD KGTLDAVLTD EEEKTLQQVD RMLAEVGRVL QVGGRYLCIS LAQAHILKKA VGHFSREGWM VRVHQVANSQ DQVLEAEPQF SLPVFAFIMT KFRPVPGSAL QIFELCAQEQ RKPVRLESAE RLAEAVQERQ QYAWLCSQLR RKARLGSVSL DLCDGDTGEP RYTLHVVDSP TVKPSRDNHF AIFIIPQGRE TEWLFGMDEG RKQLAASAGF RRLITVALHR GQQYESMDHI QAELSARVME LAPAGMPTQQ QVPFLSVGGD IGVRTVQHQD CSPLSGDYVI EDVQGDDKRY FRRLIFLSNR NVVQSEARLL KDVSHKAQKK RKKDRKKQRP ADAEDLPAAP GQSIDKSYLC CEHHKAMIAG LALLRNPELL LEIPLALLVV GLGGGSLPLF VHDHFPKSCI DAVEIDPSML EVATQWFGFS QSDRMKVHIA DGLDYIASLA GGGEARPCYD VIMFDVDSKD PTLGMSCPPP AFVEQSFLQK VKSILTPEGV FILNLVCRDL GLKDSVLAGL KAVFPLLYVR RIEGEVNEIL FCQLHPEQKL ATPELLETAQ ALERTLRKPG RGWDDTYVLS DMLKTVKIV Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Methyltransferase Like 13 (METTL13) (AA 1-699) protein (GST tag)
METTL13 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Methyltransferase Like 13 (METTL13) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)METTL13 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- METTL13 (Methyltransferase Like 13 (METTL13))
- Alternative Name
- METTL13 (METTL13 Products)
- Background
- EEF1A lysine and N-terminal methyltransferase (eEF1A-KNMT) (Methyltransferase-like protein 13) [Includes: eEF1A lysine methyltransferase (EC 2.1.1.-), eEF1A N-terminal methyltransferase (EC 2.1.1.-)],FUNCTION: Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation (PubMed:30612740, PubMed:30143613). Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2' (PubMed:30143613). Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55' (PubMed:30612740, PubMed:30143613). Has no activity towards core histones H2A, H2B, H3 and H4 (PubMed:30612740). Negatively regulates cell proliferation at G1/S transition via transcriptional suppression of cell cycle regulatory genes such as CDK4 and CDK6 (PubMed:26763933). {ECO:0000269|PubMed:26763933, ECO:0000269|PubMed:30143613, ECO:0000269|PubMed:30612740}.
- Molecular Weight
- 78.8 kDa
- UniProt
- Q8N6R0
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