CYB5R2 Protein (AA 1-276) (His tag)
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- Target See all CYB5R2 Proteins
- CYB5R2 (Cytochrome B5 Reductase 2 (CYB5R2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-276
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CYB5R2 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant CYB5R2 Protein expressed in mammalian cells.
- Sequence
- MNSRRREPIT LQDPEAKYPL PLIEKEKISH NTRRFRFGLP SPDHVLGLPV GNYVQLLAKI DNELVVRAYT PVSSDDDRGF VDLIIKIYFK NVHPQYPEGG KMTQYLENMK IGETIFFRGP RGRLFYHGPG NLGIRPDQTS EPKKTLADHL GMIAGGTGIT PMLQLIRHIT KDPSDRTRMS LIFANQTEED ILVRKELEEI ARTHPDQFNL WYTLDRPPIG WKYSSGFVTA DMIKEHLPPP AKSTLILVCG PPPLIQTAAH PNLEKLGYTQ DMIFTY Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Cytochrome B5 Reductase 2 (CYB5R2) (AA 1-276) protein (His tag)
custom-made CYB5R2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cytochrome B5 Reductase 2 (CYB5R2) (AA 1-276) protein (His tag)CYB5R2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Cytochrome B5 Reductase 2 (CYB5R2) (AA 1-237) protein (GST tag)CYB5R2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cytochrome B5 Reductase 2 (CYB5R2) protein (Myc-DYKDDDDK Tag)CYB5R2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- CYB5R2 (Cytochrome B5 Reductase 2 (CYB5R2))
- Alternative Name
- CYB5R2 (CYB5R2 Products)
- Background
- NADH-cytochrome b5 reductase 2 (b5R.2) (EC 1.6.2.2),FUNCTION: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1). {ECO:0000250, ECO:0000269|PubMed:15858218}.
- Molecular Weight
- 31.5 kDa
- UniProt
- Q6BCY4
- AlphaFold
- Q6BCY4
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