NOTO Protein (AA 1-251) (His tag)
custom-made
NOTO
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all NOTO products
- NOTO (Notochord Homeobox (NOTO))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-251
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NOTO protein is labelled with His tag.
- Purpose
- Made-to-order recombinant NOTO Protein expressed in mammalian cells.
- Sequence
- MPSPRPRGSP PPAPSGSRVR PPRSGRSPAP RSPTGPNTPR APGRFESPFS VEAILARPDP CAPAASQPSG SACVHPAFWT AASLCATGGL PWACPTSWLP AYLSVGFYPV PGPRVAPVCG LLGFGVTGLE LAHCSGLWAF PDWAPTEDLQ DTERQQKRVR TMFNLEQLEE LEKVFAKQHN LVGKKRAQLA ARLKLTENQV RVWFQNRRVK YQKQQKLRAA VTSAEAASLD EPSSSSIASI QSDDAESGVD G Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Notochord Homeobox (NOTO) (AA 1-240) protein (His tag)
custom-made NOTO Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- NOTO (Notochord Homeobox (NOTO))
- Alternative Name
- NOTO (NOTO Products)
- Background
- Homeobox protein notochord,FUNCTION: Transcription regulator acting downstream of both FOXA2 and Brachyury (T) during notochord development. Required for node morphogenesis. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning, acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate. Activates the transcription of ciliary proteins C11orf97 homolog, FAM183B and SPACA9 in the embryonic ventral node (By similarity). {ECO:0000250|UniProtKB:Q5TIS6}.
- Molecular Weight
- 27.0 kDa
- UniProt
- A8MTQ0
- AlphaFold
- A8MTQ0
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