POU3F1 Protein (AA 1-451) (His tag)
custom-made
POU3F1
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all POU3F1 Proteins
- POU3F1 (POU Class 3 Homeobox 1 (POU3F1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-451
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This POU3F1 protein is labelled with His tag.
- Purpose
- Custom-made recombinant POU3F1 Protein expressed in mammalian cells.
- Sequence
- MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAAAERL HAGAAYREVQ KLMHHEWLGA GAGHPVGLAH PQWLPTGGGG GGDWAGGPHL EHGKAGGGGT GRADDGGGGG GFHARLVHQG AAHAGAAWAQ GSTAHHLGPA MSPSPGASGG HQPQPLGLYA QAAYPGGGGG GLAGMLAAGG GGAGPGLHHA LHEDGHEAQL EPSPPPHLGA HGHAHGHAHA GGLHAAAAHL HPGAGGGGSS VGEHSDEDAP SSDDLEQFAK QFKQRRIKLG FTQADVGLAL GTLYGNVFSQ TTICRFEALQ LSFKNMCKLK PLLNKWLEET DSSSGSPTNL DKIAAQGRKR KKRTSIEVGV KGALESHFLK CPKPSAHEIT GLADSLQLEK EVVRVWFCNR RQKEKRMTPA AGAGHPPMDD VYAPGELGPG GGGASPPSAP PPPPPAALHH HHHHTLPGSV Q Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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POU Class 3 Homeobox 1 (POU3F1) (AA 1-449) protein (His tag)
custom-made POU3F1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- POU3F1 (POU Class 3 Homeobox 1 (POU3F1))
- Alternative Name
- POU3F1 (POU3F1 Products)
- Background
- POU domain, class 3, transcription factor 1 (Octamer-binding protein 6) (Oct-6) (Octamer-binding transcription factor 6) (OTF-6) (POU domain transcription factor SCIP),FUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). Acts as a transcriptional repressor of myelin-specific genes (By similarity). {ECO:0000250|UniProtKB:P20267, ECO:0000250|UniProtKB:P21952}.
- Molecular Weight
- 45.5 kDa
- UniProt
- Q03052
- AlphaFold
- Q03052
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