CARD18 Protein (AA 1-90) (His tag)
-
- Target See all CARD18 Proteins
- CARD18 (Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-90
- Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CARD18 protein is labelled with His tag.
- Purpose
- Custom-made recombinant CARD18 Protein expressed in mammalian cells.
- Sequence
- MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL VTGKGPKSCC KFIKHLCEED PQLASKMGLH Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
-
Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
-
Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18) (AA 1-90) protein (His tag)
CARD18 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18) (AA 1-90) protein (GST tag)CARD18 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18) protein (Myc-DYKDDDDK Tag)CARD18 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
-
- Target
- CARD18 (Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18))
- Alternative Name
- CARD18 (CARD18 Products)
- Background
- Caspase recruitment domain-containing protein 18 (Caspase-1 inhibitor Iceberg),FUNCTION: Inhibits generation of IL-1-beta by interacting with caspase-1 and preventing its association with RIP2. Down-regulates the release of IL1B. {ECO:0000269|PubMed:11051551}.
- Molecular Weight
- 10.1 kDa
- UniProt
- P57730
- AlphaFold
- P57730
-
