CDCA3 Protein (AA 1-268) (His tag)
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- Target See all CDCA3 Proteins
- CDCA3 (Cell Division Cycle Associated 3 (CDCA3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-268
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CDCA3 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant CDCA3 Protein expressed in mammalian cells.
- Sequence
- MGSAKSVPVT PARPPPHNKH LARVADPRSP SAGILRTPIQ VESSPQPGLP AGEQLEGLKH AQDSDPRSPT LGIARTPMKT SSGDPPSPLV KQLSEVFETE DSKSNLPPEP VLPPEAPLSS ELDLPLGTQL SVEEQMPPWN QTEFPSKQVF SKEEARQPTE TPVASQSSDK PSRDPETPRS SGSMRNRWKP NSSKVLGRSP LTILQDDNSP GTLTLRQGKR PSPLSENVSE LKEGAILGTG RLLKTGGRAW EQGQDHDKEN QHFPLVES Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Cell Division Cycle Associated 3 (CDCA3) (AA 1-266) protein (His tag)
custom-made CDCA3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cell Division Cycle Associated 3 (CDCA3) (AA 1-268) protein (GST tag)CDCA3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cell Division Cycle Associated 3 (CDCA3) protein (Myc-DYKDDDDK Tag)CDCA3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- CDCA3 (Cell Division Cycle Associated 3 (CDCA3))
- Alternative Name
- CDCA3 (CDCA3 Products)
- Background
- Cell division cycle-associated protein 3 (Gene-rich cluster protein C8) (Trigger of mitotic entry protein 1) (TOME-1),FUNCTION: F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity). {ECO:0000250}.
- Molecular Weight
- 29.0 kDa
- UniProt
- Q99618
- AlphaFold
- Q99618
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