DENND4B Protein (AA 1-1496) (His tag)
custom-made
DENND4B
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all DENND4B products
- DENND4B (DENN/MADD Domain Containing 4B (DENND4B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1496
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DENND4B protein is labelled with His tag.
- Purpose
- Custom-made recombinant DENND4B Protein expressed in mammalian cells.
- Sequence
- MAEERPPRLV DYFVVAGLAG NGAPIPEETW VPEPSGPLRP PRPAEPITDV AVIARALGEE VPQGYTCIQA SAGGHPLELS AGLLGGTQPV ICYRRGRDKP PLVELGVLYE GKERPKPGFQ VLDTTPYSHS ANLAPPGPGH PRTYLTYRRA AEGAGLHALG ITDLCLVLPS KGEGTPHTYC RLPRNLNPGM WGPAVYLCYK VGLAKANTLV YEAELLGRYP EEDNEAFPLP ESVPVFCLPM GATIECWPAQ TKYPVPVFST FVLTGAAGDK VYGAALQFYE AFPRARLSER QARALGLLSA VERGRALGGR AVRSRRAIAV LSRWPAFPAF RAFLTFLYRY SVSGPHRLPL EAHISHFIHN VPFPSPQRPR ILVQMSPYDN LLLCQPVSSP LPLSGASFLQ LLQSLGPELA ITLLLAVLTE HKLLVHSLRP DLLTSVCEAL VSMIFPLHWQ CPYIPLCPLV LADVLSAPVP FIVGIHSSYF DLHDPPADVI CVDLDTNTLF QTEEKKLLSP RTLPRRPYKV LLATLTNLYQ QLDQTYTGPE EEASLEFLLT DYEAVCGRRA RLEREVQGAF LRFMACLLKG YRVFLRPLTQ APSEGARDVD NLFFLQGFLK SRERSSHKLY SQLLHTQMFS QFIEECSFGS ARHAALEFFD SCVEKVHPEQ EKPEPTPLVE LEELSGSELT VFITPPEEPA LPEGSESTPQ YCYDGFPELR AELFESLQEQ PGALPVPGPS RSAPSSPAPR RTKQEMKVAQ RMAQKSAAVP ELWARCLLGH CYGLWFLCLP AYVRSAPSRV QALHTAYHVL RQMESGKVVL PDEVCYRVLM QLCSHYGQPV LSVRVMLEMR QAGIVPNTIT YGYYNKAVLE SKWPSGTPGG RLRWAKLRNV VLGAAQFRQP LRERQQQQQQ QQQQQQQQQQ EQVSAHQEAG SSQAEPYLER PSPTRPLQRQ TTWAGRSLRD PASPPGRLVK SGSLGSARGA QPTVEAGVAH MIEALGVLEP RGSPVPWHDG SLSDLSLTGE EPLPGGSPGG SGSALSAQST EALEGLSGRG PKAGGRQDEA GTPRRGLGAR LQQLLTPSRH SPASRIPPPE LPPDLPPPAR RSPMDSLLHP RERPGSTASE SSASLGSEWD LSESSLSNLS LRRSSERLSD TPGSFQSPSL EILLSSCSLC RACDSLVYDE EIMAGWAPDD SNLNTTCPFC ACPFVPLLSV QTLDSRPSVP SPKSAGASGS KDAPVPGGPG PVLSDRRLCL ALDEPQLCNG HMGGASRRVE SGAWAYLSPL VLRKELESLV ENEGSEVLAL PELPSAHPII FWNLLWYFQR LRLPSILPGL VLASCDGPSH SQAPSPWLTP DPASVQVRLL WDVLTPDPNS CPPLYVLWRV HSQIPQRVVW PGPVPASLSL ALLESVLRHV GLNEVHKAVG LLLETLGPPP TGLHLQRGIY REILFLTMAA LGKDHVDIVA FDKKYKSAFN KLASSMGKEE LRHRRAQMPT PKAIDCRKCF GAPPEC Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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DENN/MADD Domain Containing 4B (DENND4B) (AA 1-1499) protein (His tag)
custom-made DENND4B Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
DENN/MADD Domain Containing 4B (DENND4B) protein (Myc-DYKDDDDK Tag)DENND4B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- DENND4B (DENN/MADD Domain Containing 4B (DENND4B))
- Alternative Name
- DENND4B (DENND4B Products)
- Background
- DENN domain-containing protein 4B,FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. {ECO:0000269|PubMed:20937701}.
- Molecular Weight
- 163.8 kDa
- UniProt
- O75064
- AlphaFold
- O75064
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