PHF17 Protein (AA 1-842) (His tag)

Catalog No. ABIN7554196

Product Details

Target: PHF17 (PHD Finger Protein 17 (PHF17))

Protein Type: Recombinant

Protein Characteristics: AA 1-842

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PHF17 protein is labelled with His tag.

Purpose: Custom-made recombinant JADE1 Protein expressed in mammalian cells.

Sequence: MKRGRLPSSS EDSDDNGSLS TTWSQNSRSQ HRRSSCSRHE DRKPSEVFRT DLITAMKLHD SYQLNPDEYY VLADPWRQEW EKGVQVPVSP GTIPQPVARV VSEEKSLMFI RPKKYIVSSG SEPPELGYVD IRTLADSVCR YDLNDMDAAW LELTNEEFKE MGMPELDEYT MERVLEEFEQ RCYDNMNHAI ETEEGLGIEY DEDVVCDVCQ SPDGEDGNEM VFCDKCNICV HQACYGILKV PEGSWLCRTC ALGVQPKCLL CPKKGGAMKP TRSGTKWVHV SCALWIPEVS IGSPEKMEPI TKVSHIPSSR WALVCSLCNE KFGASIQCSV KNCRTAFHVT CAFDRGLEMK TILAENDEVK FKSYCPKHSS HRKPEESLGK GAAQENGAPE CSPRNPLEPF ASLEQNREEA HRVSVRKQKL QQLEDEFYTF VNLLDVARAL RLPEEVVDFL YQYWKLKRKV NFNKPLITPK KDEEDNLAKR EQDVLFRRLQ LFTHLRQDLE RVRNLTYMVT RREKIKRSVC KVQEQIFNLY TKLLEQERVS GVPSSCSSSS LENMLLFNSP SVGPDAPKIE DLKWHSAFFR KQMGTSLVHS LKKPHKRDPL QNSPGSEGKT LLKQPDLCGR REGMVVPESF LGLEKTFAEA RLISAQQKNG VVMPDHGKRR DNRFHCDLIK GDLKDKSFKQ SHKPLRSTDV SQRHLDNTRA ATSPGVGQSA PGTRKEIVPK CNGSLIKVNY NQTAVKVPTT PASPVKNWGG FRIPKKGERQ QQGEAHDGAC HQHSDYPYLG LGRVPAKERA KSKLKSDNEN DGYVPDVEMS DSESEASEKK CIHTSSTISR RTDIIRRSIL AS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PHF17 (PHD Finger Protein 17 (PHF17))

Alternative Name: JADE1 (PHF17 Products)

Background: Protein Jade-1 (Jade family PHD finger protein 1) (PHD finger protein 17),FUNCTION: Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (PubMed:16387653, PubMed:19187766, PubMed:20129055, PubMed:24065767). Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac), regulating DNA replication initiation, regulating DNA replication initiation (PubMed:20129055, PubMed:24065767). May also promote acetylation of nucleosomal histone H4 by KAT5 (PubMed:15502158). Promotes apoptosis (PubMed:16046545). May act as a renal tumor suppressor (PubMed:16046545). Negatively regulates canonical Wnt signaling, at least in part, cooperates with NPHP4 in this function (PubMed:22654112). {ECO:0000269|PubMed:15502158, ECO:0000269|PubMed:16046545, ECO:0000269|PubMed:16387653, ECO:0000269|PubMed:19187766, ECO:0000269|PubMed:20129055, ECO:0000269|PubMed:22654112, ECO:0000269|PubMed:24065767}.

Molecular Weight: 95.5 kDa

UniProt: Q6IE81

AlphaFold: Q6IE81