NOL8 Protein (AA 1-1167) (His tag)
custom-made
NOL8
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all NOL8 products
- NOL8 (Nucleolar Protein 8 (NOL8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1167
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NOL8 protein is labelled with His tag.
- Purpose
- Custom-made recombinant NOL8 Protein expressed in mammalian cells.
- Sequence
- MKVNRETKRL YVGGLSQDIS EADLQNQFSR FGEVSDVEII TRKDDQGNPQ KVFAYINISV AEADLKKCMS VLNKTKWKGG TLQIQLAKES FLHRLAQERE AAKAKKEEST TGNANLLEKT GGVDFHMKAV PGTEVPGHKN WVVSKFGRVL PVLHLKNQHK RKIIKYDPSK YCHNLKKIGE DFSNTIPISS LTWELEGGND PMSKKRRGEF SDFHGPPKKI IKVQKDESST GSLAMSTRPR RVIERPPLTQ QQAAQKRTCD SITPSKSSPV PVSDTQKLKN LPFKTSGLET AKKRNSISDD DTDSEDELRM MIAKEENLQR TTQPSINESE SDPFEVVRDD FKSGVHKLHS LIGLGIKNRV SCHDSDDDIM RNDREYDSGD TDEIIAMKKN VAKVKNSTEF SQMEKSTKKT SFKNRENCEL SDHCIKLQKR KSNVESALSH GLKSLNRKSP SHSSSSEDAD SASELADSEG GEEYNAMMKN CLRVNLTLAD LEQLAGSDLK VPNEDTKSDG PETTTQCKFD RGSKSPKTPT GLRRGRQCIR PAEIVASLLE GEENTCGKQK PKENNLKPKF QAFKGVGCLY EKESMKKSLK DSVASNNKDQ NSMKHEDPSI ISMEDGSPYV NGSLGEVTPC QHAKKANGPN YIQPQKRQTT FESQDRKAVS PSSSEKRSKN PISRPLEGKK SLSLSAKTHN IGFDKDSCHS TTKTEASQEE RSDSSGLTSL KKSPKVSSKD TREIKTDFSL SISNSSDVSA KDKHAEDNEK RLAALEARQK AKEVQKKLVH NALANLDGHP EDKPTHIIFG SDSECETEET STQEQSHPGE EWVKESMGKT SGKLFDSSDD DESDSEDDSN RFKIKPQFEG RAGQKLMDLQ SHFGTDDRFR MDSRFLETDS EEEQEEVNEK KTAEEEELAE EKKKALNVVQ SVLQINLSNS TNRGSVAAKK FKDIIHYDPT KQDHATYERK RDDKPKESKA KRKKKREEAE KLPEVSKEMY YNIAMDLKEI FQTTKYTSEK EEGTPWNEDC GKEKPEEIQD PAALTSDAEQ PSGFTFSFFD SDTKDIKEET YRVETVKPGK IVWQEDPRLQ DSSSEEEDVT EETDHRNSSP GEASLLEKET TRFFFFSKND ERLQGSDLFW RGVGSNMSRN SWEARTTNLR MDCRKKHKDA KRKMKPK Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- NOL8 (Nucleolar Protein 8 (NOL8))
- Alternative Name
- NOL8 (NOL8 Products)
- Background
- Nucleolar protein 8 (Nucleolar protein Nop132),FUNCTION: Plays an essential role in the survival of diffuse-type gastric cancer cells. Acts as a nucleolar anchoring protein for DDX47. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells. {ECO:0000269|PubMed:14660641, ECO:0000269|PubMed:15132771, ECO:0000269|PubMed:16963496}.
- Molecular Weight
- 131.6 kDa
- UniProt
- Q76FK4
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