SCAF4 Protein (AA 1-1147) (His tag)
custom-made
SCAF4
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all SCAF4 products
- SCAF4 (SR-Related CTD-Associated Factor 4 (SCAF4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1147
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SCAF4 protein is labelled with His tag.
- Purpose
- Custom-made recombinant SCAF4 Protein expressed in mammalian cells.
- Sequence
- MDAVNAFNQE LFSLMDMKPP ISRAKMILIT KAAIKAIKLY KHVVQIVEKF IKKCKPEYKV PGLYVIDSIV RQSRHQFGTD KDVFGPRFSK NITATFQYLY LCPSEDKSKI VRVLNLWQKN GVFKIEIIQP LLDMAAGTSN AAPVAENVTN NEGSPPPPVK VSSEPPTQAT PNSVPAVPQL PSSDAFAAVA QLFQTTQGQQ LQQILQTFQQ PPKPQSPALD NAVMAQVQAI TAQLKTTPTQ PSEQKAAFPP PEQKTAFDKK LLDRFDYDDE PEAVEESKKE DTTAVTTTAP AAAVPPAPTA TVPAAAAPAA ASPPPPQAPF GFPGDGMQQP AYTQHQNMDQ FQPRMMGIQQ DPMHHQVPLP PNGQMPGFGL LPTPPFPPMA QPVIPPTPPV QQPFQASFQA QNEPLTQKPH QQEMEVEQPC IQEVKRHMSD NRKSRSRSAS RSPKRRRSRS GSRSRRSRHR RSRSRSRDRR RHSPRSRSQE RRDREKERER RQKGLPQVKP ETASVCSTTL WVGQLDKRTT QQDVASLLEE FGPIESINMI PPRGCAYIVM VHRQDAYRAL QKLSRGNYKV NQKSIKIAWA LNKGIKADYK QYWDVELGVT YIPWDKVKPE ELESFCEGGM LDSDTLNPDW KGIPKKPENE VAQNGGAETS HTEPVSPIPK PLPVPVPPIP VPAPITVPPP QVPPHQPGPP VVGALQPPAF TPPLGIPPPG FGPGVPPPPP PPPFLRPGFN PMHLPPGFLP PGPPPPITPP VSIPPPHTPP ISIPNSTIAG INEDTTKDLS IGNPIPTVVS GARGNAESGD SVKMYGSAVP PAAPTNLPTP PVTQPVSLLG TQGVAPGPVI GLQAPSTGLL GARPGLIPLQ RPPGMPPPHL QRFPLMPPRP MPPHMMHRGP PPGPGGFAMP PPHGMKGPFP PHGPFVRPGG MPGLGGPGPG PGGPEDRDGR QQPPQQPQQQ PQPQAPQQPQ QQQQQQPPPS QQPPPTQQQP QQFRNDNRQQ FNSGRDQERF GRRSFGNRVE NDRERYGNRN DDRDNSNRDR REWGRRSPDR DRHRDLEERN RRSSGHRDRE RDSRDRESRR EKEEARGKEK PEVTDRAGGN KTVEPPISQV GNVDTASELE KGVSEAAVLK PSEELPAEAT SSVEPEKDSG SAAEAPR Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- SCAF4 (SR-Related CTD-Associated Factor 4 (SCAF4))
- Alternative Name
- SCAF4 (SCAF4 Products)
- Background
- SR-related and CTD-associated factor 4 (CTD-binding SR-like protein RA4) (Splicing factor, arginine/serine-rich 15),FUNCTION: Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF8, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF8, also acts as a suppressor of transcriptional readthrough (PubMed:31104839). {ECO:0000269|PubMed:31104839}.
- Molecular Weight
- 125.9 kDa
- UniProt
- O95104
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