SPIRE2 Protein (AA 1-714) (His tag)
custom-made
SPIRE2
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all SPIRE2 Proteins
- SPIRE2 (Spire Homolog 2 (SPIRE2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-714
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SPIRE2 protein is labelled with His tag.
- Purpose
- Custom-made recombinant SPIRE2 Protein expressed in mammalian cells.
- Sequence
- MARAGSCGGA AAGAGRPEPW ELSLEEVLKA YEQPLNEEQA WAVCFQGCRG LRGSPGRRLR DTGDLLLRGD GSVGAREPEA AEPATMVVPL ASSEAQTVQS LGFAIYRALD WGLDESEERE LSPQLERLID LMANNDSEDS GCGAADEGYG GPEEEEEAEG VPRSVRTFAQ AMRLCAARLT DPRGAQAHYQ AVCRALFVET LELRAFLARV REAKEMLQKL REDEPHLETP RAELDSLGHT DWARLWVQLM RELRRGVKLK KVQEQEFNPL PTEFQLTPFE MLMQDIRARN YKLRKVMVDG DIPPRVKKDA HELILDFIRS RPPLKQVSER RLRPLPPKQR SLHEKILEEI KQERRLRPVR GEGWAARGFG SLPCILNACS GDAKSTSCIN LSVTDAGGSA QRPRPRVLLK APTLAEMEEM NTSEEEESPC GEVTLKRDRS FSEHDLAQLR SEVASGLQSA THPPGGTEPP RPRAGSAHVW RPGSRDQGTC PASVSDPSHP LLSNRGSSGD RPEASMTPDA KHLWLEFSHP VESLALTVEE VMDVRRVLVK AEMEKFLQNK ELFSSLKKGK ICCCCRAKFP LFSWPPSCLF CKRAVCTSCS IKMKMPSKKF GHIPVYTLGF ESPQRVSAAK TAPIQRRDIF QSLQGPQWQS VEEAFPHIYS HGCVLKDVCS ECTSFVADVV RSSRKSVDVL NTTPRRSRQT QSLYIPNTRT LDFK Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Spire Homolog 2 (SPIRE2) (AA 1-718) protein (His tag)
custom-made SPIRE2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Spire Homolog 2 (SPIRE2) protein (Myc-DYKDDDDK Tag)SPIRE2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- SPIRE2 (Spire Homolog 2 (SPIRE2))
- Alternative Name
- SPIRE2 (SPIRE2 Products)
- Background
- Protein spire homolog 2 (Spir-2),FUNCTION: Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (By similarity). Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480). {ECO:0000250|UniProtKB:Q8K1S6, ECO:0000269|PubMed:21620703, ECO:0000269|PubMed:26287480}.
- Molecular Weight
- 79.7 kDa
- UniProt
- Q8WWL2
- AlphaFold
- Q8WWL2
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