TYW1B Protein (AA 1-668) (His tag)
custom-made
TYW1B
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all TYW1B products
- TYW1B (tRNA-YW Synthesizing Protein 1 Homolog B (S. Cerevisiae) (TYW1B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-668
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TYW1B protein is labelled with His tag.
- Purpose
- Custom-made recombinant TYW1B Protein expressed in mammalian cells.
- Sequence
- MDPSADTWDL SSPLISLWIN RFYIYLGFAV SISLWICVQI VIEMQGFATV LAEAVTSLDL PVAIINLKEY DPDDHLIEEV TSKNVCVFLV ATYTDGLPTE SAEWFCKWLE EASIDFRFGK TYLKGMRDAV FGLGNSAYAS HFNKVGKNVD KWLWMLGVHR VMSRGEGDCD VVKSKHGSIE ANFRAWKTKF ISQLQALQKG ERKKSCGGHC KKGKCESHQH GSEEREEGSQ EQDELHHRDT KEEEPFESSS EEEFGGEDHQ SLNSIVDVED LGKIMDHVKK EKREKEQQEE KSGLFRNMGR NEDGERRAMI TPALREALTK QVDAPRERSL LQTHILWNES HRCMETTPSL ACANKCVFCW WHHNNPVGTE WLWKMDQPEM ILKEAIENHQ NMIKQFKGVP GVKAERFEEG MTVKHCALSL VGEPIMYPEI NRFLKLLHQC KISSFLVTNA QFPAEIRNLE PVTQLYVSVD ASTKDSLKKI DRPLFKDFWQ QFLDSLKALA VKQQRTVYRL MLVKAWNVDE LQAYAQLVSL GNPDFIEVKG VTYCRESSAS SLTMAHVPWH EEVVQFVREL VDLIPEYEIA CEHEHSNCLL IAHRKFKIGG EWWTWIDYNR FQELIQEYED SGGSKTFSAK DYMARTPHWA LFGANERSFD PKDTRHQRKN KSKAISGC Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- TYW1B (tRNA-YW Synthesizing Protein 1 Homolog B (S. Cerevisiae) (TYW1B))
- Alternative Name
- TYW1B (TYW1B Products)
- Background
- S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1B (EC 4.1.3.44) (Radical S-adenosyl methionine and flavodoxin domain-containing protein 2) (tRNA wybutosine-synthesizing protein 1 homolog B),FUNCTION: Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis (By similarity). {ECO:0000250}.
- Molecular Weight
- 76.9 kDa
- UniProt
- Q6NUM6
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