ZRANB3 Protein (AA 1-1079) (His tag)
custom-made
ZRANB3
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all ZRANB3 products
- ZRANB3 (Zinc Finger, RAN-Binding Domain Containing 3 (ZRANB3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1079
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ZRANB3 protein is labelled with His tag.
- Purpose
- Custom-made recombinant ZRANB3 Protein expressed in mammalian cells.
- Sequence
- MPRVHNIKKS LTPHISCVTN ESDNLLDFLP DRLRAKLLPF QKDGIIFALK RNGRCMVADE MGLGKTIQAI GITYFYKEEW PLLIVVPSSL RYPWTEEIEK WIPELSPEEI NVIQNKTDVR RMSTSKVTVL GYGLLTADAK TLIDALNNQN FKVVIVDESH YMKSRNATRS RILLPIVQKA RRAILLTGTP ALGRPEELFM QIEALFPQKF GRWTDYAKRY CNAHIRYFGK RPQWDCRGAS NLNELHQLLS DIMIRRLKTE VLTQLPPKVR QRIPFDLPSA AAKELNTSFE EWEKIMRTPN SGAMETVMGL ITRMFKQTAI AKAGAVKDYI KMMLQNDSLK FLVFAHHLSM LQACTEAVIE NKTRYIRIDG SVSSSERIHL VNQFQKDPDT RVAILSIQAA GQGLTFTAAS HVVFAELYWD PGHIKQAEDR AHRIGQCSSV NIHYLIANGT LDTLMWGMLN RKAQVTGSTL NGRKEKIQAE EGDKEKWDFL QFAEAWTPND SSEELRKEAL FTHFEKEKQH DIRSFFVPQP KKRQLMTSCD ESKRFREENT VVSSDPTKTA ARDIIDYESD VEPETKRLKL AASEDHCSPS EETPSQSKQI RTPLVESVQE AKAQLTTPAF PVEGWQCSLC TYINNSELPY CEMCETPQGS AVMQIDSLNH IQDKNEKDDS QKDTSKKVQT ISDCEKQALA QSEPGQLADS KEETPKIEKE DGLTSQPGNE QWKSSDTLPV YDTLMFCASR NTDRIHIYTK DGKQMSCNFI PLDIKLDLWE DLPASFQLKQ YRSLILRFVR EWSSLTAMKQ RIIRKSGQLF CSPILALEEI TKQQTKQNCT KRYITKEDVA VASMDKVKNV GGHVRLITKE SRPRDPFTKK LLEDGACVPF LNPYTVQADL TVKPSTSKGY LQAVDNEGNP LCLRCQQPTC QTKQACKANS WDSRFCSLKC QEEFWIRSNN SYLRAKVFET EHGVCQLCNV NAQELFLRLR DAPKSQRKNL LYATWTSKLP LEQLNEMIRN PGEGHFWQVD HIKPVYGGGG QCSLDNLQTL CTVCHKERTA RQAKERSQVR RQSLASKHGS DITRFLVKK Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- ZRANB3 (Zinc Finger, RAN-Binding Domain Containing 3 (ZRANB3))
- Alternative Name
- ZRANB3 (ZRANB3 Products)
- Background
- DNA annealing helicase and endonuclease ZRANB3 (Annealing helicase 2) (AH2) (Zinc finger Ran-binding domain-containing protein 3) [Includes: DNA annealing helicase ZRANB3 (EC 3.6.4.-), Endonuclease ZRANB3 (EC 3.1.-.-)],FUNCTION: DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558). {ECO:0000269|PubMed:21078962, ECO:0000269|PubMed:22704558, ECO:0000269|PubMed:22705370, ECO:0000269|PubMed:22759634, ECO:0000269|PubMed:26884333}.
- Molecular Weight
- 123.2 kDa
- UniProt
- Q5FWF4
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