NEK3 Protein (AA 1-511) (His tag)
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- Target See all NEK3 Proteins
- NEK3 (NIMA related kinase 3 (NEK3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-511
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NEK3 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Nek3 Protein expressed in mammalian cells.
- Sequence
- MDNYTVLRVI GQGSFGRALL VLQESSNQTF AMKEIRLLKS DTQTSRKEAV LLAKMKHPNI VAFKESFEAE GYLYIVMEYC DGGDLMQRIK QQKGKLFPED TILNWFIQIC LGVNHIHKRR VLHRDIKSKN VFLTHNGKVK LGDFGSARLL SSPMAFACTY VGTPYYVPPE IWENLPYNNK SDIWSLGCIL YELCALKHPF QANSWKNLIL KICQGPIHPL PALYSCKLQG LVKQMLKRNP SHRPSATTLL CRGSLAPLVP KCLPPQIIRE YGEQILDEIK ISTPKNMKKQ DSNRVRRALG EANSASMQEE ERGRKCSHTE LESTGTTPAG NALERAARGN PESGNPQEHG RHTSPASPHR PWWERHGPSS NVEALEKASI LTSSFTAEDD RGGSVIKYEE NARRQWVREP PEALLSMLKD ADLSQAFQTY TIYRPGAEGF LKGPLSEDTA SDSVDGDLDS VMLDPERFEP RLDEEDTDFE EDNENPDWVS ELKKHVGYGD GPGGQLLGER A Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product NEK3 Protein
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NIMA related kinase 3 (NEK3) (AA 1-506) protein (His tag)
custom-made NEK3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NIMA related kinase 3 (NEK3) (AA 1-506) protein (GST tag)NEK3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
NIMA related kinase 3 (NEK3) (Active) protein (GST tag,His tag)NEK3 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE Active
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- NEK3 (NIMA related kinase 3 (NEK3))
- Alternative Name
- Nek3 (NEK3 Products)
- Background
- Serine/threonine-protein kinase Nek3 (EC 2.7.11.1) (Never in mitosis A-related kinase 3) (NimA-related protein kinase 3),FUNCTION: Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. {ECO:0000269|PubMed:19509051}.
- Molecular Weight
- 57.5 kDa
- UniProt
- Q9R0A5
- AlphaFold
- Q9R0A5
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