TCF7L1 Protein (AA 1-584) (His tag)

Catalog No. ABIN7556382

Product Details

Target: TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))

Protein Type: Recombinant

Protein Characteristics: AA 1-584

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TCF7L1 protein is labelled with His tag.

Purpose: Custom-made recombinant Tcf7l1 Protein expressed in mammalian cells.

Sequence: MPQLGGGRGG AGGGGGGSGA GATSGGDDLG ANDELIPFQD EGGEEQEPSS DTASAQRDLD EVKSSLVNES ENQSSSSDSE AERRPQPARD AFQKPRDYFA EVRRPQDGAF FKGGAYPGYP FLMIPDLSSP YLSNGPLSPG GARTYLQMKW PLLDVPSSAT VKDTRSPSPA HLSNKVPVVQ HPHHMHPLTP LITYSNDHFS PASPPTHLSP EIDPKTGIPR PPHPSELSPY YPLSPGAVGQ IPHPLGWLVP QQGQPMYSLP PGGFRHPYPA LAMNASMSSL VSSRFPHMVA PAHPGLPTSG IPHPAIVSPI VKQEPAAPSL SPAVSAKSPV TVKKEEEKKP HVKKPLNAFM LYMKEMRAKV VAECTLKESA AINQILGRKW HNLSREEQAK YYELARKERQ LHAQLYPTWS ARDNYGKKKK RKREKQLSQT QSQQQIQEAE GALASKSKKP CIQYLPPEKP CDSPASSHGS ALDSPATPSA ALASPAAPAA THSEQAQPLS LTTKPEARAQ LALHSAAFLS AKAAASNSSQ MGSQPPLLSR PLPLGSMPAA LLTSPPTFPA TLHAHQALPV LQAQPLSLVT KSAH Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))

Alternative Name: Tcf7l1 (TCF7L1 Products)

Background: Transcription factor 7-like 1 (HMG box transcription factor 3) (TCF-3) (mTCF-3),FUNCTION: Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. {ECO:0000269|PubMed:11445543}.

Molecular Weight: 62.3 kDa

UniProt: Q9Z1J1

AlphaFold: Q9Z1J1

Pathways: WNT Signaling, Stem Cell Maintenance