ARIH2 Protein (AA 1-492) (His tag)

Catalog No. ABIN7556384

Product Details

Target: ARIH2 (protein ariadne-2 homolog (ARIH2))

Protein Type: Recombinant

Protein Characteristics: AA 1-492

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ARIH2 protein is labelled with His tag.

Purpose: Custom-made recombinant Arih2 Protein expressed in mammalian cells.

Sequence: MSVDMNSQGS DSNEEDYDPN CEEEEEEEED PGDIEDYYVG VASDVEQQGA DAFDPEEYQF TCLTYKESEG ALHEHMTSLA SVLKVSHSVA KLILVNFHWQ VSEILDRYRS NSAQLLVEAR VQPNPSKHVP TAHPPHHCAV CMQFVRKENL LSLACQHQFC RSCWEQHCSV LVKDGVGVGI SCMAQDCPLR TPEDFVFPLL PNEELRDKYR RYLFRDYVES HFQLQLCPGA DCPMVIRVQE PRARRVQCNR CSEVFCFKCR QMYHAPTDCA TIRKWLTKCA DDSETANYIS AHTKDCPKCN ICIEKNGGCN HMQCSKCKHD FCWMCLGDWK THGSEYYECS RYKENPDIVN QSQQAQAREA LKKYLFYFER WENHNKSLQL EAQTYERIHE KIQERVMNNL GTWIDWQYLQ NAAKLLAKCR YTLQYTYPYA YYMESGPRKK LFEYQQAQLE AEIENLSWKV ERADSYDRGD LENQMHIAEQ RRRTLLKDFH DT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ARIH2 (protein ariadne-2 homolog (ARIH2))

Alternative Name: Arih2 (ARIH2 Products)

Background: E3 ubiquitin-protein ligase ARIH2 (ARI-2) (Protein ariadne-2 homolog) (EC 2.3.2.31) (RING-type E3 ubiquitin transferase ARIH2) (Triad1 protein) (UbcM4-interacting protein 48),FUNCTION: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (By similarity). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity). The initial ubiquitin is then elongated (By similarity). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex. Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination. May play a role in myelopoiesis (By similarity). {ECO:0000250|UniProtKB:O95376, ECO:0000250|UniProtKB:Q9Y4X5}.

Molecular Weight: 57.7 kDa

UniProt: Q9Z1K6

AlphaFold: Q9Z1K6