DACT3 Protein (AA 1-610) (His tag)
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- Target See all DACT3 products
- DACT3 (Dapper, Antagonist of beta-Catenin, Homolog 3 (DACT3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-610
- Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DACT3 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Dact3 Protein expressed in mammalian cells.
- Sequence
- MIRAFSFPVS PERGRLRGWL EGSLAGLCEL HWLRERQEYR VQQALRLAQP GMGGAEAEDE EDAEEDEDAA AARRAAAALE EQLEALPGLI WDLGQQLGDL SLESGGLDQE SGRSSGFYED PSSTGGPDSP PSTFCGDSGF SGSGSYGRLG PSDPRGIYAS ERPKSLGDAS PSAPESVGAR VAVPRSFSAP YPTAAAGAET CSSAERRARA GPFLTPSPLH AVALRSPRPS GRVPCGSPDG AASRPLDGYI SALLRRRRRR GAGQPRTSPG GADGGARRQN GARPRPPEAS PPPGGARPAR EPSTERAWAA AWEAEVPPEP APPAAASPPS SPAEGRLVKA QYIPGAPAAS RGLPGRAARR RAPPLTRGRS VEQSPPRERP RAAGRRGRLA EPSGRRGSPR ARKAARSQSE TSLLGRAHAA PPPKYPTAER DEPRPPRPRR GPAPTPTVQA CRRWRSTAEI DAPDGRRPRA RVPAPRGPAP SPSAPPRRLL YGCAGSDSEC SAVGRPVPLG RRMPSGCAPG GYGESESSAS EGESPAFSSA SSDSDGSGGL VWPQQLVAAA GASPSGPGGA AGGGTPAGPA KVFVKIKASH ALKKKILRFR SGSLKVMTTV Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- DACT3 (Dapper, Antagonist of beta-Catenin, Homolog 3 (DACT3))
- Alternative Name
- Dact3 (DACT3 Products)
- Background
- Dapper homolog 3 (Dapper antagonist of catenin 3),FUNCTION: May be involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins (By similarity). {ECO:0000250}.
- Molecular Weight
- 63.3 kDa
- UniProt
- Q0PHV7
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