Lamin B Receptor Protein (LBR) (AA 1-626) (His tag)

Catalog No. ABIN7557157

Product Details

Target: Lamin B Receptor (LBR)

Protein Type: Recombinant

Protein Characteristics: AA 1-626

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This Lamin B Receptor protein is labelled with His tag.

Purpose: Custom-made recombinant Lbr Protein expressed in mammalian cells.

Sequence: MPSRKFVEGE VVRGRWPGSS LYYEVEILSH DNKSQLYTVK YKDGTELELK ESDIKPLKSF KQRKSGSISS SPSRRRGSRS RSRSRSRSRS PGRAPKGSRR SVSASHEGDV KEKKEKEMRR EILQVKLTPL VLKPFGNSVS VYNGEPEHME KNATPYKDKQ ERIILSTEDR YIVTQYSLRP RREEVKAKEI ESEEQNLVTK GPAPLGTFQV TTPQRKDLEF GGVPGAVLIM LGLPACVLLL LLQCRQKDPG LLHFPPPLPA LHELWEPRVC GVYLLWFFVQ ALFHLLPVGK VAEGTPLVDG RRLQYRLNGL YAFILTSAAL GAAVFWGVEL CYLYTHFLQL ALAATGFSVL LSAYLYVRSL RAPREELSPA SSGNAVYDFF IGRELNPRLG AFDLKFFCEL RPGLIGWVVI NLVMLLMEMK IQERAAPSLA MILVNSFQLL YVVDALWNEE ALLTSMDIMH DGFGFMLAFG DLVWVPFTYS LQAFYLVSHP HDLSWPLASV IIALKLCGYV IFRCANSQKN AFRKNPTDPK LAHLKTIHTS TGKSLLVSGW WGFVRHPNYL GDLIMALAWS LPCGFNHLLP YFYIIYFTAL LIHREARDEH QCRRKYGLAW EKYCQRVPYR IFPYIY Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Lamin B Receptor (LBR)

Alternative Name: Lbr (LBR Products)

Background: Delta(14)-sterol reductase LBR (Delta-14-SR) (EC 1.3.1.70) (3-beta-hydroxysterol Delta (14)-reductase) (C-14 sterol reductase) (C14SR) (Integral nuclear envelope inner membrane protein) (Lamin-B receptor) (Sterol C14-reductase),FUNCTION: Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis (PubMed:18785926). Plays a critical role in myeloid cell cholesterol biosynthesis which is essential to both myeloid cell growth and functional maturation (PubMed:22140257). Mediates the activation of NADPH oxidases, perhaps by maintaining critical levels of cholesterol required for membrane lipid raft formation during neutrophil differentiation (PubMed:22140257). Anchors the lamina and the heterochromatin to the inner nuclear membrane (By similarity). {ECO:0000250|UniProtKB:Q14739, ECO:0000269|PubMed:18785926, ECO:0000269|PubMed:22140257}.

Molecular Weight: 71.4 kDa

UniProt: Q3U9G9