KCTD21 Protein (AA 1-260) (His tag)
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- Target See all KCTD21 products
- KCTD21 (Potassium Channel Tetramerisation Domain Containing 21 (KCTD21))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-260
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This KCTD21 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Kctd21 Protein expressed in mammalian cells.
- Sequence
- MSDPITLNVG GKLYTTSLAT LTSFPDSMLG AMFSGKMPTK RDSQGNCFID RDGKVFRYIL NFLRTSHLDL PEDFQEMGLL RREADFYQVQ PLIEALQEKE VELSKAEKNA MLNITLKQRV QTVHFTVREA PQIYSLSSSS MEVFNANIFS TSCLFLKLLG SKLLYCSNGN LSSITSHLQD PNHLTLDWVA NVEGLPEEEY TKQNLKRLWV VPANKQINSF QVFVEEVLKI ALSDGFCIDS SHPHALDFMN NKIIRLIRYR Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Potassium Channel Tetramerisation Domain Containing 21 (KCTD21) (AA 1-260) protein (His tag)
custom-made KCTD21 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Potassium Channel Tetramerisation Domain Containing 21 (KCTD21) protein (Myc-DYKDDDDK Tag)KCTD21 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- KCTD21 (Potassium Channel Tetramerisation Domain Containing 21 (KCTD21))
- Alternative Name
- Kctd21 (KCTD21 Products)
- Background
- BTB/POZ domain-containing protein KCTD21 (KCASH2 protein) (Potassium channel tetramerization domain-containing protein 21),FUNCTION: Probable substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes the ubiquitination of HDAC1. Can function as antagonist of the Hedgehog pathway by affecting the nuclear transfer of transcription factor GLI1, the function probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. Inhibits cell growth and tumorigenicity of medulloblastoma (MDB). {ECO:0000250|UniProtKB:Q4G0X4}.
- Molecular Weight
- 29.6 kDa
- UniProt
- Q3URF8
- AlphaFold
- Q3URF8
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