C14orf45 Protein (AA 1-533) (His tag)
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- Target See all C14orf45 (CCDC176) products
- C14orf45 (CCDC176) (Coiled-Coil Domain Containing 176 (CCDC176))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-533
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This C14orf45 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Bbof1 Protein expressed in mammalian cells.
- Sequence
- MPAKDKRKDK RKDKRKGKNK GKEPKKIIKS DEPAIERAKA NASLWEARLE VTELSRIEYR DTSRRLAKNN EDLKKQQYNM EKDIMSVLSY LKKQDQEKDN MIEKLKQQLA KTKEKAQEEK EKLEQKYALQ VSELEGQFHQ KAKEIGMIQT ELKTIKQFQK RKIQVEKELD DLKENLRNSE KNYQETLRRL ESRFFEEKHR LEQEAEKRII MLAERAHHEA VVQLNTAGRN VFKENVYLHK ALAYHLKEAE ILQQNSKKIE ENHSCLLQQK EINDLLVKEK IMQLTQQKSQ IQTLQKKVVS LENALTYMTT EFEAEVLKLQ QKAIIENQAG LVEIDKLQQL LQMKDREMNR VKRLAKNILD ERTEVEQFFL DALYEVKQQI LASRKHYKQI AQAAFNLKMR AACAGKTEYP RIRTFDGKEQ STNSVNQDLL EAEKWPTTQK NVDIRDLTWE QKEKVLRLLF AKMNGFAARK YSQSSKPPVP DHIIYGSGEM KETGDENNLL DQTFITQQAP VSDSNRMVSP DVIPQGLQDS DIA Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Coiled-Coil Domain Containing 176 (CCDC176) (AA 1-529) protein (His tag)
custom-made CCDC176 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Coiled-Coil Domain Containing 176 (CCDC176) protein (His tag)CCDC176 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- C14orf45 (CCDC176) (Coiled-Coil Domain Containing 176 (CCDC176))
- Alternative Name
- Bbof1 (CCDC176 Products)
- Background
- Basal body-orientation factor 1 (Coiled-coil domain-containing protein 176),FUNCTION: Plays an essential role in sperm motility and male fertility by stabilizing the sperm flagellar axonemal structure. May be required for the stability of ODF2 and MANS1 proteins. Dispensable for the assembly and function of motile cilia. {ECO:0000269|PubMed:37198331}.
- Molecular Weight
- 62.5 kDa
- UniProt
- Q3V079
- AlphaFold
- Q3V079
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