MYST2 Protein (AA 1-613) (His tag)

Catalog No. ABIN7557544

Product Details

Target: MYST2 (MYST Histone Acetyltransferase 2 (MYST2))

Protein Type: Recombinant

Protein Characteristics: AA 1-613

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This MYST2 protein is labelled with His tag.

Purpose: Custom-made recombinant Kat7 Protein expressed in mammalian cells.

Sequence: MAIGVVKRNA GSSSDGTEDS DFSTDLEHTD SSESDGTSRR SARVTRSSAR LSQSSQDSSP VRNLPSFGTE EPAYSTRRVT RSQQQPTPVT PKKYPLRQTR SSGSETEQVV DFSDRETKNT ADHDESPPRT PTGNAPSSES DIDISSPNVS HDESIAKDMS LKDSGSDLSH RPKRRRFHES YNFNMKCPTP GCNSLGHLTG KHERHFSISG CPLYHNLSAD ECKVRAQSRD KQIEERMLSH RQDDNNRHAT RHQAPTERQL RYKEKVAELR KKRNSGLSKE QKEKYMEHRQ TYGNTREPLL ENLTSEYDLD LFRRAQARAS EDLEKLRLQG QITEGSNMIK TIAFGRYELD TWYHSPYPEE YARLGRLYMC EFCLKYMKSQ TILRRHMAKC VWKHPPGDEI YRKGSISVFE VDGKKNKIYC QNLCLLAKLF LDHKTLYYDV EPFLFYVMTE ADNTGCHLIG YFSKEKNSFL NYNVSCILTM PQYMRQGYGK MLIDFSYLLS KVEEKVGSPE RPLSDLGLIS YRSYWKEVLL RYLHNFQGKE ISIKEISQET AVNPVDIVST LQALQMLKYW KGKHLVLKRQ DLIDEWIAKE AKRSNSNKTM DPSCLKWTPP KGT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MYST2 (MYST Histone Acetyltransferase 2 (MYST2))

Alternative Name: Kat7 (MYST2 Products)

Background: Histone acetyltransferase KAT7 (EC 2.3.1.48) (Histone acetyltransferase binding to ORC1) (Lysine acetyltransferase 7) (MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2) (MYST-2),FUNCTION: Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby regulating various processes, such as gene transcription, protein ubiquitination, immune regulation, stem cell pluripotent and self-renewal maintenance and embryonic development (PubMed:21753189, PubMed:21149574, PubMed:23319590, PubMed:27733580, PubMed:31827282). Some complexes also catalyze acetylation of histone H4 at 'Lys-5', 'Lys-8' and 'Lys-12' (H4K5ac, H4K8ac and H4K12ac, respectively), regulating DNA replication initiation, regulating DNA replication initiation (By similarity). Specificity of the HBO1 complexes is determined by the scaffold subunit: complexes containing BRPF scaffold (BRPF1, BRD1/BRPF2 or BRPF3) direct KAT7/HBO1 specificity towards H3K14ac, while complexes containing JADE (JADE1, JADE2 and JADE3) scaffold direct KAT7/HBO1 specificity towards histone H4 (By similarity). H3K14ac promotes transcriptional elongation by facilitating the processivity of RNA polymerase II (PubMed:31827282). Acts as a key regulator of hematopoiesis by forming a complex with BRD1/BRPF2, directing KAT7/HBO1 specificity towards H3K14ac and promoting erythroid differentiation (By similarity). H3K14ac is also required for T-cell development (PubMed:27733580). KAT7/HBO1-mediated acetylation facilitates two consecutive steps, licensing and activation, in DNA replication initiation: H3K14ac facilitates the activation of replication origins, and histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac) facilitates chromatin loading of MCM complexes, promoting DNA replication licensing (By similarity). Acts as a positive regulator of centromeric CENPA assembly: recruited to centromeres and mediates histone acetylation, thereby preventing centromere inactivation mediated by SUV39H1, possibly by increasing histone turnover/exchange (By similarity). Involved in nucleotide excision repair: phosphorylation by ATR in response to ultraviolet irradiation promotes its localization to DNA damage sites, where it mediates histone acetylation to facilitate recruitment of XPC at the damaged DNA sites (By similarity). Acts as an inhibitor of NF-kappa-B independently of its histone acetyltransferase activity (By similarity). {ECO:0000250|UniProtKB:O95251, ECO:0000269|PubMed:21149574, ECO:0000269|PubMed:21753189, ECO:0000269|PubMed:23319590, ECO:0000269|PubMed:27733580, ECO:0000269|PubMed:31827282}., FUNCTION: Plays a central role in the maintenance of leukemia stem cells in acute myeloid leukemia (AML) (PubMed:31827282). Acts by mediating acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby facilitating the processivity of RNA polymerase II to maintain the high expression of key genes, such as HOXA9 and HOXA10 that help to sustain the functional properties of leukemia stem cells (PubMed:31827282). {ECO:0000269|PubMed:31827282}.

Molecular Weight: 70.6 kDa

UniProt: Q5SVQ0

AlphaFold: Q5SVQ0