HSD3B5 Protein (AA 1-373) (His tag)
custom-made
HSD3B5
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all HSD3B5 products
- HSD3B5 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta and Steroid delta-Isomerase 5 (HSD3B5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-373
- Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HSD3B5 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant Hsd3b5 Protein expressed in mammalian cells.
- Sequence
- MPGWSCLVTG AGGFLGQRIV RMLVQEEELQ EIRALFRTFG RKHEEELSKL QTKAKVRVLK GDILDAQCLK RACQGMSAVI HTAAAIDPLG AASRQTILDV NLKGTQLLLD ACVEASVPTF IYSSSVLVAG PNSYKEIILN AHEEEHREST WPNPYPYSKR MAEKAVLATN GRLLKNGGTL HTCALRLPFI YGEECQVTST TVKTALKNNS IIKKNATFSI ANPVYVGNAA WAHILAARSL QDPKKSPSIQ GQFYYISDNT PHQSYDDLNY TLSKEWGLCL DSGWSLPLSL LYWLAFLLET VSFLLRPVYN YRPPFNRLLI TVLNSVFTIS YKKAQRDLGY QPLVSWEEAK QKTSEWIGTL VKQHRETLHK KSQ Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- HSD3B5 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta and Steroid delta-Isomerase 5 (HSD3B5))
- Alternative Name
- Hsd3b5 (HSD3B5 Products)
- Background
- NADPH-dependent 3-keto-steroid reductase Hsd3b5 (3 beta-hydroxysteroid dehydrogenase type 5) (3 beta-hydroxysteroid dehydrogenase type V) (3 beta-HSD V) (EC 1.1.1.270) (Dihydrotestosterone 3-ketoreductase) (EC 1.1.1.210),FUNCTION: Responsible for the reduction of the oxo group on the C-3 of 5alpha-androstane steroids. Catalyzes the conversion of dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Does not function as an isomerase. {ECO:0000269|PubMed:7491113}.
- Molecular Weight
- 41.9 kDa
- UniProt
- Q61694
- AlphaFold
- Q61694
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