TRIM45 Protein (AA 1-580) (His tag)

Catalog No. ABIN7558070

Product Details

Target: TRIM45 (Tripartite Motif Containing 45 (TRIM45))

Protein Type: Recombinant

Protein Characteristics: AA 1-580

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TRIM45 protein is labelled with His tag.

Purpose: Custom-made recombinant Trim45 Protein expressed in mammalian cells.

Sequence: MSEIRKPLLG FVHKLQDANA SGSSGKTHCP TCLRLFKVPR LLPCLHTVCT TCLEKLDPFS VVDIRGGDSD TSSEGSVFQD PELCSLQPQI GILCPVCDAQ VDLPLGGVKA LTVDHLAMND VLLENLRGEG QGLVCDLCSD REVEKRCQTC KANLCHFCCQ AHRRQKKTTY HTMVDLKDLK GYSQVGKPIL CPSHPAEELR LFCELCDRPV CRDCVVGEHR EHPYDFTSNV IHKHGDSVRE LLRDTQPHVE ALEDALAQIK SVNNALQERV EAVAADVRTF SEGYIKAIEE HRDKLLQQLD DIRIQRETAL QLQKAQLEQL LADMRTGVEF TEHLLTSGSD LEILITKGVV VERLRKLNKV EYSARPGVNH KICFSPQEKA GQCQGYEVYG AINTQEVDPA QCVLQGEDLH RAREKQTASF TLFCKDASGQ SMGRGGDNVH VEVVPKDKKD SPIRTVVQDN KDGSYRVSYT PKEPGIYTVW VCIREQHVQG SPFNVTVRRK HRPHPGVFHC CTFCSSGGQK AARCACGGTM PGGYLGCGHG HKGHPGRPHW SCCGKFIEKS ECSFTSGQGA PRSLLRTVAL Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM45 (Tripartite Motif Containing 45 (TRIM45))

Alternative Name: Trim45 (TRIM45 Products)

Background: E3 ubiquitin-protein ligase TRIM45 (EC 2.3.2.27) (RING finger protein 99) (Tripartite motif-containing protein 45),FUNCTION: E3 ubiquitin-protein ligase that plays a role in the regulation of inflammatory response (PubMed:36681779). Mechanistically, mediates the 'Lys-48'-linked polyubiquitination of TAB2, a regulatory protein of the kinase TAK1, leading to its degradation via the proteasomal pathway and inhibition of the TLR-mediated inflammatory immune response. May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway. {ECO:0000250|UniProtKB:Q9H8W5, ECO:0000269|PubMed:36681779}.

Molecular Weight: 64.3 kDa

UniProt: Q6PFY8

AlphaFold: Q6PFY8