ARHGEF4 Protein (AA 1-484) (His tag)

Catalog No. ABIN7558237

Product Details

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Protein Type: Recombinant

Protein Characteristics: AA 1-484

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ARHGEF4 protein is labelled with His tag.

Purpose: Custom-made recombinant Arhgef4 Protein expressed in mammalian cells.

Sequence: MDDQELGFKA GDVIEVMDAT NREWWWGRVA DGEGWFPASF VRLRVNQDEP ADDYEAPRAG AREADDSGPE AQSCKDQMRT NVINEILSTE RDYIKHLRDI CEGYVRQCRK REDMFSEEQL RTIFGNIEDI YRCQKAFVKA LEQKFNTERP HLSELGACFL EHQADFQIYS EYCNNHPNAC VELSRLTKLS KYVYFFEACR LLQRMIDISL DGFLLTPVQK ICKYPLQLAE LLKYTHPQHR DFKNVEAALH AMKNVAQLIN ERKRRLENID KIAQWQSSIE DWEGEDLLVR SSELIHSGEL TRVTQPQARS QQRMFFLFDR QLIYCKKDLL RRDVLYYKGR LDMDDLEVVD VEDGKDRDLH VSVKNAFRLY CGTTGDSHLL CARKPEQKQR WLKAFARERE QVRLDQETGF SITELQRKQA MLNASKQQAT GKPKAVGRPG YLTRHKHPSL PASRPQQQVL VLAEPRRKPS NFWHSISRLA PFRK Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Alternative Name: Arhgef4 (ARHGEF4 Products)

Background: Rho guanine nucleotide exchange factor 4 (APC-stimulated guanine nucleotide exchange factor) (Asef),FUNCTION: Acts as a guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion (By similarity). Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. {ECO:0000250, ECO:0000269|PubMed:19893577}.

Molecular Weight: 56.7 kDa

UniProt: Q7TNR9

Pathways: Neurotrophin Signaling Pathway