PEF1 Protein (AA 1-275) (His tag)

Catalog No. ABIN7558506

Product Details

Target: PEF1 (Penta-EF-Hand Domain Containing 1 (PEF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-275

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This PEF1 protein is labelled with His tag.

Purpose: Made-to-order recombinant Pef1 Protein expressed in mammalian cells.

Sequence: MASYPNGQSC PGAAGQVPGV PPGGYYPGPP HGGGQYGSGL PPGGGYGAPA PGGPYGYPSA GGVPSGTPSG PYGGIPPGGP YGQLPPGGPY GTQPGHYGQG GVPPNVDPEA YSWFQSVDAD HSGYISLKEL KQALVNSNWS SFNDETCLMM INMFDKTKSG RIDVAGFSAL WKFLQQWRNL FQQYDRDRSG SISSTELQQA LSQMGYNLSP QFTQLLVSRY CARSAIPAMQ LDCFIKVCTQ LQVLTEAFRE KDTAVQGNIR LSFEDFVTMT ASRML Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PEF1 (Penta-EF-Hand Domain Containing 1 (PEF1))

Alternative Name: Pef1 (PEF1 Products)

Background: Peflin (PEF protein with a long N-terminal hydrophobic domain) (Penta-EF hand domain-containing protein 1),FUNCTION: Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (By similarity). Also acts as a negative regulator of ER-Golgi transport, possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity). {ECO:0000250|UniProtKB:Q641Z8, ECO:0000250|UniProtKB:Q9UBV8}.

Molecular Weight: 29.2 kDa

UniProt: Q8BFY6

AlphaFold: Q8BFY6