Paxx Protein (AA 1-205) (His tag)
custom-made
Paxx
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all Paxx products
- Paxx (Non-homologous End Joining Factor (Paxx))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-205
- Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Paxx protein is labelled with His tag.
- Purpose
- Custom-made recombinant Paxx Protein expressed in mammalian cells.
- Sequence
- MAPPLLSLPL CILPPGSGSP RLVCYCERDS GGDGDRDDFN LYVTDAAELW STCFSPDSLA RLKARFGLSG AEDIHSRFRA ACQQQAVTVS LQEDRALITL SGDTPALAFD LSKVPSPEAA PRLQALTLSL AEHVCNLERR LAAAEETITS PKKNTQPAGT QFLPELDHQR GSSGPGVRRR CPGESLINPG FKSKKPAAGV DFDET Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Paxx (Non-homologous End Joining Factor (Paxx))
- Alternative Name
- Paxx (Paxx Products)
- Background
- Protein PAXX (Paralog of XRCC4 and XLF),FUNCTION: Non-essential DNA repair protein involved in DNA non-homologous end joining (NHEJ), participates in double-strand break (DSB) repair and V(D)J recombination (PubMed:27601299, PubMed:27798842, PubMed:27601633, PubMed:27830975, PubMed:28051062, PubMed:29077092). May act as a scaffold required for accumulation of the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites and promote the assembly and/or stability of the NHEJ machinery (PubMed:28051062). Involved in NHEJ by promoting the ligation of blunt-ended DNA ends (By similarity). Together with NHEJ1/XLF, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends (By similarity). Constitutes a non-essential component of classical NHEJ: has a complementary but distinct function with NHEJ1/XLF in DNA repair (PubMed:27601299, PubMed:27798842, PubMed:27830975, PubMed:28051062). {ECO:0000250|UniProtKB:Q9BUH6, ECO:0000269|PubMed:27601299, ECO:0000269|PubMed:27601633, ECO:0000269|PubMed:27798842, ECO:0000269|PubMed:27830975, ECO:0000269|PubMed:28051062, ECO:0000269|PubMed:29077092}.
- Molecular Weight
- 22.0 kDa
- UniProt
- Q8K0Y7
- AlphaFold
- Q8K0Y7
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