TSPAN33 Protein (AA 1-283) (His tag)

Catalog No. ABIN7559746

Product Details

Target: TSPAN33 (Tetraspanin 33 (TSPAN33))

Protein Type: Recombinant

Protein Characteristics: AA 1-283

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TSPAN33 protein is labelled with His tag.

Purpose: Custom-made recombinant Tspan33 Protein expressed in mammalian cells.

Sequence: MARRPGVPAA YGDEFSFVSP LVKYLLFFFN MLFWVISMVM VAVGVYARLM KHAEAALACL AVDPAILLIV VGVLMFLLTF CGCIGSLREN ICLLQTFSLC LTIVFLLQLA AGILGFVFSD KARGKVSEII NNAIVHYRDD LDLQNLIDFG QKKFSCCGGI SYRDWSQNMY FNCSEDNPSR ERCSVPYSCC LPTPNQAVIN TMCGQGMQAL DYLEASKVIY TNGCIDKLVN WIHSNLFLLG GVALGLAIPQ LVGILLSQVL VNQIKDQIKL QLYNQQHRAD PWY Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TSPAN33 (Tetraspanin 33 (TSPAN33))

Alternative Name: Tspan33 (TSPAN33 Products)

Background: Tetraspanin-33 (Tspan-33) (Penumbra) (mPen) (Proerythroblast new membrane),FUNCTION: Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates (By similarity). Plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors (PubMed:17158226). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (By similarity). Mediates docking of ADAM10 to zonula adherens by interacting with ADAM10 and, in a PDZD11-dependent manner, with the zonula adherens protein PLEKHA7 (PubMed:30463011). {ECO:0000250|UniProtKB:Q86UF1, ECO:0000269|PubMed:17158226, ECO:0000269|PubMed:30463011}.

Molecular Weight: 31.6 kDa

UniProt: Q8R3S2