CDKAL1 Protein (AA 1-578) (His tag)
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- Target See all CDKAL1 Proteins
- CDKAL1 (CDK5 Regulatory Subunit Associated Protein 1-Like 1 (CDKAL1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-578
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CDKAL1 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant Cdkal1 Protein expressed in mammalian cells.
- Sequence
- MPSASCDVLL DDIEDIISQE DSKPQDRQFS RKHVFPKVRR RNTQKYLQEE PRPPSDSTIP GIQKIWIRTW GCSHNNSDGE YMAGQLAAYG YKITENASDA DLWLLNSCTV KNPAEDHFRN SIKKAQEENK KVVLAGCVPQ AQPRQDYLKG LSIIGVQQID RVVEVVEETI KGHSVRLLGQ KKDNGKRLGG ARLDLPKIRK NPLIEIISIN TGCLNACTYC KTKHARGNLA SYPIDELVER AKQSFQEGVC EIWLTSEDTG AYGRDIGTDL PTLLWKLVEV IPEGAMLRLG MTNPPYILEH LEEMAKILNH PRVYAFLHIP VQSASDSVLM DMKREYCVAD FKRVVDFLKE KVPGITIATD IICGFPGETD QDFQETVKLV EEYKFPSLFI NQFYPRPGTP AAKAEQVPAH VKKQRTKDLS RVFHSYNPYD HKIGERQQVL VTEESFDSKF YVAHNRFYEQ VLVPKNPAFM GKMVEVDIYE SGKHFLKGQP VSETRVYTPS ISKPLAKGEV SGLTKEFRNR LGNHPNGTSD TCPATQHGSA YSRMVLQMSQ YDCALKVATG LALLALLLHF WPDSLLTM Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product CDKAL1 Protein
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- CDKAL1 (CDK5 Regulatory Subunit Associated Protein 1-Like 1 (CDKAL1))
- Alternative Name
- Cdkal1 (CDKAL1 Products)
- Background
- Threonylcarbamoyladenosine tRNA methylthiotransferase (EC 2.8.4.5) (CDK5 regulatory subunit-associated protein 1-like 1) (tRNA-t(6)A37 methylthiotransferase),FUNCTION: Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. {ECO:0000269|PubMed:20584901}.
- Molecular Weight
- 65.3 kDa
- UniProt
- Q91WE6
- AlphaFold
- Q91WE6
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