RMND5B Protein (AA 1-393) (His tag)
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- Target See all RMND5B products
- RMND5B (Required For Meiotic Nuclear Division 5 Homolog B (RMND5B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-393
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RMND5B protein is labelled with His tag.
- Purpose
- Custom-made recombinant Rmnd5b Protein expressed in mammalian cells.
- Sequence
- MEQCACVERE LDKVLHKFLT YGQHCEQSLE ELLHSVGQLR AELASAALQG TPLSATLSLV MSQCCRKIRD TVQKLASDHK DIHSSVSRVG KAIDRNFDSE ICGVVSDAVW DSREKQQQIL QMAIVEHLYQ QGMLSVAEEL CQESTLNVDL DFKQPFLELN RILEALHEQD LGPALEWAVS HRQRLLELNS SLEFKLHRLH FIRLLAGGPE KQLEALSYAR HFQPFARLHQ REIQVMMGSL VYLRLGLEKS PYCHLLDNSH WAEICETFTR DACSLLGLSV ESPLSVSFAS GCVALPVLMN IKAVIEQRQC TGVWSHKDEL PIEIELGMKC WYHSVFACPI LRQQTSDSNP PIKLICGHVI SRDALNKLIN GGKLKCPYCP MEQNPADGKR IIF Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Required For Meiotic Nuclear Division 5 Homolog B (RMND5B) (AA 1-393) protein (His tag)
custom-made RMND5B Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Required For Meiotic Nuclear Division 5 Homolog B (RMND5B) protein (Myc-DYKDDDDK Tag)RMND5B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- RMND5B (Required For Meiotic Nuclear Division 5 Homolog B (RMND5B))
- Alternative Name
- Rmnd5b (RMND5B Products)
- Background
- E3 ubiquitin-protein transferase RMND5B (EC 2.3.2.27) (Protein RMD5 homolog B),FUNCTION: Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex. Catalytic activity of the complex is required for normal cell proliferation. The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1. {ECO:0000250|UniProtKB:Q96G75}.
- Molecular Weight
- 44.4 kDa
- UniProt
- Q91YQ7
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