ATPBD3 Protein (AA 1-420) (His tag)
custom-made
CTU1
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all ATPBD3 (CTU1) Proteins
- ATPBD3 (CTU1) (Cytosolic Thiouridylase Subunit 1 Homolog (CTU1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-420
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ATPBD3 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Ctu1 Protein expressed in mammalian cells.
- Sequence
- MPAPTCFSCH KTRAALRRPR SGQALCGSCF CAAFEAEVLH TVLAGHLLPP GAVVAVGASG GKDSTVLAHV LRELAPRLGI TLHLVAVDEG IGGYRDAALE AVRSQAARWE LPLTIVAYED LFGGWTMDAV ARSTAGSGRS RSCCTFCGVL RRRALEEGAR LVGATHIVTG HNADDMAETV LMNFLRGDAG RLARGGVLGS TGEGCALPRC RPLQFASQKE VVLYAHFRHL RYFSEECVYA PEAFRGHARD LLKLLEAARP SAVLDLVHSA ERLALAPAAK PPPPGTCSRC GALASHKLCQ ACALLDGLNR GLPRLAIGKG RRVLQVEPPQ PGNPSLVTSD PVAPAGPCTC KQPKDKANPC GNGGDRAGAT CVSQCDLSPG NGEDRAGATC VSQRDLSLGN GGDRAGATCV SQCDLSPVAE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- ATPBD3 (CTU1) (Cytosolic Thiouridylase Subunit 1 Homolog (CTU1))
- Alternative Name
- Ctu1 (CTU1 Products)
- Background
- Cytoplasmic tRNA 2-thiolation protein 1 (EC 2.7.7.-) (ATP-binding domain-containing protein 3) (Cytoplasmic tRNA adenylyltransferase 1),FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. {ECO:0000255|HAMAP-Rule:MF_03053}.
- Molecular Weight
- 43.8 kDa
- UniProt
- Q99J10
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