NMNAT3 Protein (AA 1-245) (His tag)

Catalog No. ABIN7560317

Product Details

Target: NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))

Protein Type: Recombinant

Protein Characteristics: AA 1-245

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This NMNAT3 protein is labelled with His tag.

Purpose: Custom-made recombinant Nmnat3 Protein expressed in mammalian cells.

Sequence: MKNRIPVVLL ACGSFNPITN MHLRLFEVAR DHLHQTGRYQ VIEGIISPVN DSYGKKDLVA SHHRVAMARL ALQTSDWIRV DPWESEQAQW METVKVLRHH HRELLRSSAQ MDGPDPSKTP SASAALPELK LLCGADVLKT FQTPNLWKDT HIQEIVEKFG LVCVSRSGHD PERYISDSPI LQQFQHNIHL AREPVLNEIS ATYVRKALGQ GQSVKYLLPE AVITYIRDQG LYINDGSWKG KGKTG Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))

Alternative Name: Nmnat3 (NMNAT3 Products)

Background: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3 (NMN/NaMN adenylyltransferase 3) (EC 2.7.7.1) (EC 2.7.7.18) (Nicotinamide-nucleotide adenylyltransferase 3) (NMN adenylyltransferase 3) (Nicotinate-nucleotide adenylyltransferase 3) (NaMN adenylyltransferase 3),FUNCTION: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form, nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (By similarity). Protects against axonal degeneration following injury. {ECO:0000250|UniProtKB:Q96T66, ECO:0000269|PubMed:16914673}.

Molecular Weight: 27.7 kDa

UniProt: Q99JR6

AlphaFold: Q99JR6