RPUSD4 Protein (AA 1-377) (His tag)

Catalog No. ABIN7560693

Product Details

Target: RPUSD4 (RNA Pseudouridylate Synthase Domain Containing 4 (RPUSD4))

Protein Type: Recombinant

Protein Characteristics: AA 1-377

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This RPUSD4 protein is labelled with His tag.

Purpose: Custom-made recombinant Rpusd4 Protein expressed in mammalian cells.

Sequence: MAAPCLRTPG VQLLSMSSRP GRLFTPGSWS FCSSATSSRP LNAQRLAEKL RAQKQEQKAK EVRVPTNPVQ RRVQELVRFT QQLQRVHPNV LAKELSRRIL HQDNDLVVIN KPYGLPVHGG PGVQLCISDV LPILAKMLHG HKAEPLHLCH RLDKETTGVM VLAWEKDMAH QVQELFRTRQ VEKKYWAITV RVPLPSAGVV DIPIKEKEVQ GPQQHHKMTL SPSYRLDNGK MVKVRASRDA HVAVTQYQVL SSASSSALVE LQPVTGIKHQ LRVHLSFGLD CPILGDHKYS DWTRLAPQKL SAGTLKKLGL QQSKARYIPL HLHARQLILP ALGSRTEELL LTCKLPHFFA RSLLRLGLDM PNQDQSRGNK ARHVEAR Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RPUSD4 (RNA Pseudouridylate Synthase Domain Containing 4 (RPUSD4))

Alternative Name: Rpusd4 (RPUSD4 Products)

Background: Pseudouridylate synthase RPUSD4, mitochondrial (EC 5.4.99.-) (RNA pseudouridylate synthase domain-containing protein 4),FUNCTION: Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA). This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome. As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA, controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation. Acts on position 39 in mitochondrial tRNA(Phe). Also catalyzes pseudouridylation of mRNAs in nucleus: acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. {ECO:0000250|UniProtKB:Q96CM3}.

Molecular Weight: 42.4 kDa

UniProt: Q9CWX4

AlphaFold: Q9CWX4