DET1 Protein (DET1) (AA 1-550) (His tag)
custom-made
DET1
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all DET1 Proteins
- DET1
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-550
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DET1 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Det1 Protein expressed in mammalian cells.
- Sequence
- MDHHVSTIKP RRIQNQNVIH RLERRRISSG KAGTHWHQVR VFHQNVFPNF TVVNVEKPPC FLRKFSPDGR YFIAFSSDQT SLEIYEYQGC QAAEDLLQGY EGEILSNGND QRSVSIRGRL FERFFVLLHI TNVAANGEHL NRECSLFTDD CRCVIVGSAA YLPDEPHPPF YEVYRNSESV TPNPRSPLED YSLHIIDLHT GRLCDTRTFK CDKVVLSHNQ GLYLYKNILA ILSVQQQTIH VFQVTPEGTF IDVRTIGRFC YEDDLLTVSA VFPEVQRDSQ TGMANPFRDP FINSLKHRLL VYLWRRAEQD GSAMAKRRFF QYFDQLRQLR MWKMQLLDEN HLFIKYTSED VVTLRVTDPS QASFFVVYNM VTTEVIAVFE NTSDELLELF ENFCDLFRNA TLHSEVQFPC SASSNNFARQ IQRRFKDTII NAKYGGHTEA VRRLLGQLPI SAQSYSGSPY LDLSLFSYDD KWVSVMERPK TCGDHPIRFY ARDSGLLKFE IQAGLLGRPI NHTVRRLVAF TFHPFEPFAI SVQRTNAEYV VNFHMRHCCT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product DET1 Protein
-
DET1 (DET1) (AA 1-550) protein (His tag)
custom-made DET1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- DET1
- Alternative Name
- Det1 (DET1 Products)
- Background
- DET1 homolog (De-etiolated-1 homolog),FUNCTION: Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation (By similarity). {ECO:0000250}.
- Molecular Weight
- 63.8 kDa
- UniProt
- Q9D0A0
- AlphaFold
- Q9D0A0
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