DDX56 Protein (AA 1-546) (His tag)

Catalog No. ABIN7560850

Product Details

Target: DDX56 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 (DDX56))

Protein Type: Recombinant

Protein Characteristics: AA 1-546

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DDX56 protein is labelled with His tag.

Purpose: Made-to-order recombinant Ddx56 Protein expressed in mammalian cells.

Sequence: MEDQEALGFE HMGLDPRLLQ AVTDLGWSRP TLIQEKAIPL ALEGKDLLAR ARTGSGKTAA YAIPMLQSLL HKKATGPVME QAVRGLVLVP TKELARQAQA MIQQLAAYCA RDVRVANVSA AEDSASQRAV LMEKPDVVVG TPSRVLSHLQ QNTLKLRDSL ELLVVDEADL LFSFGFEDEL KSLLCHLPRI YQAFLMSATF NEDVQTLKEL VLHNPVTLKL QESQLPGPDQ LQQFQVVCET EEDKFLLLYA LLKLSLIRGK ALLFVNTLER GYRLRLFLEQ FSIPSCVLNG ELPLRSRCHI ISQFNQGLYD CVIATDAEIL GPQVKGKRRG RGSKGNKASD PESGVARGID FHHVSAVLNF DLPPTAEAYV HRAGRTARAN NPGIVLTFVL PAEQPFLGKI EDLLSGEGEA PILLPYQFQM EEIESFRYRC RDAMRSVTKQ AIREARLKEI KEELLHSEKL KTYFEDNPRD LQLLRHDLPL HPAVVKPHLG HVPDYLVPAA LRGLVHPRKK RRKVPFSRKA KKVKAQNPLR DFKHRGKKPK PAAKPS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DDX56 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 (DDX56))

Alternative Name: Ddx56 (DDX56 Products)

Background: Probable ATP-dependent RNA helicase DDX56 (EC 3.6.4.13) (ATP-dependent 61 kDa nucleolar RNA helicase) (DEAD box protein 56),FUNCTION: Nucleolar RNA helicase that plays a role in various biological processes including innate immunity, ribosome biogenesis or nucleolus organization. Plays an essential role in maintaining nucleolar integrity in planarian stem cells (PubMed:32703285). Maintains embryonic stem cells proliferation by conventional regulation of ribosome assembly and interaction with OCT4 and POU5F1 complex (PubMed:32703285). Regulates antiviral innate immunity by inhibiting the virus-triggered signaling nuclear translocation of IRF3. Mechanistically, acts by disrupting the interaction between IRF3 and importin IPO5. May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity (By similarity). {ECO:0000250|UniProtKB:Q9NY93, ECO:0000269|PubMed:32703285}.

Molecular Weight: 61.2 kDa

UniProt: Q9D0R4

AlphaFold: Q9D0R4