PHYHD1 Protein (AA 1-291) (His tag)
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- Target See all PHYHD1 Proteins
- PHYHD1 (Phytanoyl-CoA Dioxygenase Domain Containing 1 (PHYHD1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-291
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PHYHD1 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Phyhd1 Protein expressed in mammalian cells.
- Sequence
- MACLSPSQLK KFQEDGFLLL EGFFTADECV AMQQRIGEIV AEMDVPLHCR TEFSTQEDEQ LQTQGKTDYF LSSGDKIRFF FEKGVFDEKG NFLVPPEKSI NKIGHALHAH DPVFRSITHS PKVQALVRSL GLQMPVVVQS MYIFKQPHFG GEVSPHQDAT FLYTEPLGRV LGLWIAMEDA MLENGCLWFI PGSHTRGVSR RMIRAPSDSG PGTSFLGSDP AWASNLFVPL PVRRGGLVLI HGEVVHKSEQ NHSDHSRQAY TVHLMEAAGT VWSPGNWLQP TPELPFPPLY S Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product PHYHD1 Protein
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- PHYHD1 (Phytanoyl-CoA Dioxygenase Domain Containing 1 (PHYHD1))
- Alternative Name
- Phyhd1 (PHYHD1 Products)
- Background
- Phytanoyl-CoA dioxygenase domain-containing protein 1 (Protein PHYHD1) (EC 1.14.11.-),FUNCTION: 2-oxoglutarate(2OG)-dependent dioxygenase that catalyzes the conversion of 2-oxoglutarate to succinate and CO(2) in an iron-dependent manner. However, does not couple 2OG turnover to the hydroxylation of acyl-coenzyme A derivatives, implying that it is not directly involved in phytanoyl coenzyme-A metabolism. Does not show detectable activity towards fatty acid CoA thioesters. {ECO:0000250|UniProtKB:Q5SRE7}.
- Molecular Weight
- 32.5 kDa
- UniProt
- Q9DB26
- AlphaFold
- Q9DB26
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