ARHGAP27 Protein (AA 1-869) (His tag)

Catalog No. ABIN7561886

Product Details

Target: ARHGAP27 (rho GTPase Activating Protein 27 (ARHGAP27))

Protein Type: Recombinant

Protein Characteristics: AA 1-869

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ARHGAP27 protein is labelled with His tag.

Purpose: Custom-made recombinant Arhgap27 Protein expressed in mammalian cells.

Sequence: MAADVEGDVY VLVEHPFEYT GKDGRRIAIQ PNERYRLLRR STEHWWHVRR EPGGRPFYLP AQYVRELPAL GDPAPAPQPS VPQQRPAVPE PLAYDYRFVS TPVGADGSSA EPRGRASSLC GPARQRTGGQ RNSLAPGGPA CLYVRPAAPV RPAQSLDDLA RGGTAPPAGL LGSAGHFKAS SVAGSWVCPR PLAPSDSENV YEAIPDLRCP PRAESPKQVD DPPEPVYANV ERQPRATSPR SAAAPPRLSP VWETHTDTGT GRPYYYNPDT GVTTWESPFE TPEGTTSPAT SRASVGSGES LETEWGQYWD EESRRVFFYN PLTGETAWED ETEELEEDHQ EQLEMQPSLS PRSPGQQRPP TPETDYPELL ASYPEEDYSP VGSFSDPGPA SPLVAPPGWS CQITPDKQML YTNQFTQEQW VRLEDQHGKP YFYNPEDSSV QWELPQVPIP APRSVRKSSQ DSDTPAQASP PEEKIKTLDK AGVLHRTKTV DKGKRLRKKH WSTSWTVLEG GVLTFFKDSK TSAAGGLRQP SKLSTPEYTV ELKGASLSWA PKDKSSKKNV LELRSRDGSE YLIQHDSEAI ISTWHKAIAE GISELSADLL QGEEGEPSSA DFGSSERLGS WREEDVRQNA ASPSLSPGGL ESDLSRVRHK LRKFLQRRPT LQSLRDKGYI KDQVFGCALA QLCERERSPV PRFVQQCIRT VEARGLDIDG LYRISGNLAT IQKLRYKVDH DERLDLDDGR WEDVHVITGA LKLFFRELPE PLFPFSHFHQ FIAAIKLQDP AQRSRCVRDL VRTLPAPNQD TLRLLIQHLC RVIEHGEQNR MTVQNVAIVF GPTLLRPEME EASMPMTMVF QNQVVELILH QCADIFPPH Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ARHGAP27 (rho GTPase Activating Protein 27 (ARHGAP27))

Alternative Name: Arhgap27 (ARHGAP27 Products)

Background: Rho GTPase-activating protein 27 (CIN85-associated multi-domain-containing Rho GTPase-activating protein 1) (Rho-type GTPase-activating protein 27),FUNCTION: Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity). {ECO:0000250}.

Molecular Weight: 97.0 kDa

UniProt: A2AB59