PCYT1A Protein (AA 1-367) (His tag)
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- Target See all PCYT1A Proteins
- PCYT1A (Phosphate Cytidylyltransferase 1, Choline, alpha (PCYT1A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-367
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PCYT1A protein is labelled with His tag.
- Purpose
- Custom-made recombinant Pcyt1a Protein expressed in mammalian cells.
- Sequence
- MDAQSSAKVN SRKRRKEAPG PNGATEEDGI PSKVQRCAVG LRQPAPFSDE IEVDFSKPYV RVTMEEACRG TPCERPVRVY ADGIFDLFHS GHARALMQAK NLFPNTYLIV GVCSDELTHN FKGFTVMNEN ERYDAVQHCR YVDEVVRNAP WTLTPEFLAE HRIDFVAHDD IPYSSAGSDD VYKHIKDAGM FAPTQRTEGI STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKKY HLQERVDKVK KKVKDVEEKS KEFVQKVEEK SIDLIQKWEE KSREFIGSFL EMFGPEGALK HMLKEGKGRM LQAISPKQSP SSSPTHERSP SPSFRWPFSG KTSPSSSPAS LSRCRAVTCD ISEDEED Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product PCYT1A Protein
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Phosphate Cytidylyltransferase 1, Choline, alpha (PCYT1A) (AA 1-367) protein (His tag)
custom-made PCYT1A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phosphate Cytidylyltransferase 1, Choline, alpha (PCYT1A) (AA 1-367) protein (GST tag)PCYT1A Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Phosphate Cytidylyltransferase 1, Choline, alpha (PCYT1A) protein (Myc-DYKDDDDK Tag)PCYT1A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- PCYT1A (Phosphate Cytidylyltransferase 1, Choline, alpha (PCYT1A))
- Alternative Name
- Pcyt1a (PCYT1A Products)
- Background
- Choline-phosphate cytidylyltransferase A (EC 2.7.7.15) (CCT-alpha) (CTP:phosphocholine cytidylyltransferase A) (CCT A) (CT A) (Phosphorylcholine transferase A),FUNCTION: Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis. {ECO:0000269|PubMed:12842190}.
- Molecular Weight
- 41.7 kDa
- UniProt
- P49586
- AlphaFold
- P49586
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