SMG6 Protein (AA 1-1418) (His tag)
custom-made
SMG6
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all SMG6 products
- SMG6 (Smg-6 Homolog, Nonsense Mediated mRNA Decay Factor (SMG6))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1418
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SMG6 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Smg6 Protein expressed in mammalian cells.
- Sequence
- MAEGLERVRI SASELRGILA TLAPQAGSRE NMKELKEPRQ RKDNRRPDLE IYKPGLSRLR NRPKTKEASG NEEFKDEIVN DRDSSAVGND TQLIQVCKEL DSQQQNGPID AENSQAQETF PKTVGLEDRS LKIIKRSKKP DLQIYQPGRR LQTITKESAG RADEEEILNQ VEQLRIEEDE CKGEAIKEEV NNKPDKTEIE KHQSNDRVRT AKGEKGKKIE KGEGSKKVAD DSVPGKPGSV KRYSRSDKRR NRYRTCSTSS AGSNNSAEGA GLTDNGCRRR RQDRAKERPR LKKQVSLSST DSLDEDRVDE PDVLGSRRSS ERKKHLERNW SGCGEGEQKS NGKENRSALR VTFDAETMSK DSPVVRSVKD NVDRMKSDKG PSSGGKGSEK QELRHPRQEL RDRGRGILIL PAHTALSVSS SGSPESTPLG PRLLFGSGSK GSRSWGRGGT TRRLWDPNNP DQKPALKSQT PQLHFLDTDD EISPTSWGDS RQAQASYYKF QNSDNPYYYP RTPGPASQYP YAGYSPLQYP VGPTNGMYPG AYYPGYPAPS GQYVCSPLPA STMSPEEIEQ HVRNMQQQEL HRLLRVADNQ ELQLSNLLSR DRISTEGMEK MAQLRTELLQ LYERCILLDI EFSDSQNVDQ ILWKNAFYQV IEKFRQLLKD PNSENPEQIR NRLLELLDEG SDFFDSLLQK LQVTYKFKLE DYMDGLAIRS KPLRKTVKYA LISAQRSMIC QGDISRYREQ ANDTANYGKA RSWYLKAQHI APKNGRPYNQ LALLAVYTRR KLDAVYYYMR SLAASNPILT AKESLMSLFE ETKRKAEQME KKQHEEFDMS PDKWRKGKKS TFRHVGDDTT RLEIWIHPSH SRSAQGTESG KDSEQENGLG SLSPSDLNKR FILSFLHAHG KLFTRIGMET FPAVAEKVLK EFQVLLQHSP SPIGSTRMLQ LMTINMFAVH NSQLKDCFSE ECRSVIQEQA ASLGLAMFSL LVQRCTCLLK DSAKAQLSSP EDQEDQDDIK VSSFVPDLKE LLPSVKVWSD WMLGYPDTWN PPPTSLDLPL QVAVDVWSTL ADFCNILTAV NQSEVPLYKD PDDDLTLLIL EEDRLLSGFV PLLAAPQDPC YVEKTSDKVI AADCKRVTVL KYFLEALCGQ EEPLLAFKGG KYVSVAPVPD TMGKEMGSQE GKQLEDEEED VVIEDFEEDS EAEGSGGEDD IRELRAKKLA LARKIAEQQR RQEKIQAVLE DQSQMRQMEL EIRPLFLVPD TNGFIDHLAS LARLLESRKY ILVVPLIVIN ELDGLAKGQE TDHRAGGYAR VVQEKARKSI EFLERRFESR DSCLRALTSR GNELESIAFR SEDITGQLGN NDDLILSCCL HYCKDKAKDY MPTSKEEPIR LLREVVLLTD DRNLRVKALT RNVPVRDIPA FLTWAQVG Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Smg-6 Homolog, Nonsense Mediated mRNA Decay Factor (SMG6) (AA 1-1419) protein (His tag)
custom-made SMG6 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- SMG6 (Smg-6 Homolog, Nonsense Mediated mRNA Decay Factor (SMG6))
- Alternative Name
- Smg6 (SMG6 Products)
- Background
- Telomerase-binding protein EST1A (EC 3.1.-.-) (Ever shorter telomeres 1A) (Nonsense mediated mRNA decay factor SMG6) (Smg-6 homolog),FUNCTION: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER). {ECO:0000250|UniProtKB:Q86US8}., FUNCTION: Plays a role in nonsense-mediated mRNA decay. Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA. {ECO:0000250|UniProtKB:Q86US8}.
- Molecular Weight
- 160.5 kDa
- UniProt
- P61406
- AlphaFold
- P61406
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