PPP4R2 Protein (AA 1-417) (His tag)

Catalog No. ABIN7563625

Product Details

Target: PPP4R2 (Protein Phosphatase 4, Regulatory Subunit 2 (PPP4R2))

Protein Type: Recombinant

Protein Characteristics: AA 1-417

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This PPP4R2 protein is labelled with His tag.

Purpose: Custom-made recombinant Ppp4r2 Protein expressed in mammalian cells.

Sequence: MDVERLQEAL KDFEKRGKKE VCPVLDQFLC HVAKTGETMI QWSQFKGYFI FKLEKVMDDF RTSAPEPRGP PNPNVEYIPF DEMKERILKI VTGFNGIPFT IQRLCELLTD PRRNYTGTDK FLRGVEKNVM VVSCVCPSSE KNNSNSLNRM NGVMFPGNSP NYTDRSNING PGTPRPLNRP KLSLSAPLTT NGLPESTDSK DSELQLSEEK GHSDSSASES EVSLLSPVKN KHPDEDAVES EEHEVKRLKF DKEGDVRETA SQTVSGEVSS VRAEETETAA PPPDKDRESR TRQHCTEEEE EEEEEEEEEE EESFMTPREM VPERKNQEKE SDDALTVNEE TSEESHQMEG SGVSPAQTDS TSERSDSAGA SRSGSDCLET QESGGPPSSK TGESVSVPSS MESEEATEVT DDPMEQD Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PPP4R2 (Protein Phosphatase 4, Regulatory Subunit 2 (PPP4R2))

Alternative Name: Ppp4r2 (PPP4R2 Products)

Background: Serine/threonine-protein phosphatase 4 regulatory subunit 2,FUNCTION: Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair (By similarity). Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair (By similarity). {ECO:0000250}.

Molecular Weight: 46.5 kDa

UniProt: Q0VGB7

AlphaFold: Q0VGB7