SMARCA1 Protein (AA 1-1046) (His tag)

Catalog No. ABIN7564129

Product Details

Target: SMARCA1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 1 (SMARCA1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1046

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This SMARCA1 protein is labelled with His tag.

Purpose: Custom-made recombinant Smarca1 Protein expressed in mammalian cells.

Sequence: MEPDTATEAA TVAVSDARAT VVVVEDEQPG PSTFKEEGAA AAATEGTTAT EKGEKKEKIT SPFQLKLAAK ASKSEKEMDP EYEEKMVNMP LKADRAKRFE FLLKQTELFA HFIQPSAQKS PTSPLNMKLA RPRVKKDDKQ SLISVGDYRH RRTEQEEDEE LLSESRKTSN VCVRFEVSPS YVKGGPLRDY QIRGLNWLIS LYENGVNGIL ADEMGLGKTL QTIALLGYLK HYRNIPGPHM VLVPKSTLHN WMNEFKRWVP SLRVICFVGD KDVRAAFIRD EMMPGEWDVC VTSYEMVIKE KSVFKKFHWR YLVIDEAHRI KNEKSKLSEI VREFKSTNRL LLTGTPLQNN LHELWALLNF LLPDVFNSAD DFDSWFDTKN CLGDQKLVER LHAVLKPFLL RRIKTDVEKS LPPKKEIKIY LGLSKMQREW YTKILMKDID VLNSSGKMDK MRLLNILMQL RKCCNHPYLF DGAEPGPPYT TDEHIVGNSG KMVALDKLLA RIKEQGSRVL IFSQMTRLLD ILEDYCMWRG YEYSRLDGQT PHEEREEAID AFNAPNSSKF IFMLSTRAGG LGINLASADV VILYDSDWNP QVDLQAMDRA HRIGQKKPVR VFRLITDNTV EERIVERAEI KLRLDSIVIQ QGRLIDQQSN KLAKEEMLQM IRHGATHVFA CKESELTDED IVTILERGEK KTAEMNERMQ KMGESSLRNF RMDLEQSLYK FEGEDYREKQ KLGTVEWIEP PKRERKANYA VDAYFREALR VSEPKIPKAP RPPKQPNVQD FQFFPPRLFE LLEKEILYYR KTIGYKVPRN PEIPNPAIAQ REEQKKIDGA EPLTPQETEE KDKLLTQGFT NWTKRDFNQF IKANEKYGRD DIDNIAREVE GKSPEEVMEY SAVFWERCNE LQDIEKIMAQ IERGEARIQR RISIKKALDA KIARYKAPFH QLRIQYGTSK GKNYTEEEDR FLICMLHKMG FDRENVYEEL RQCVRNAPQF RFDWFIKSRT AMEFQRRCNT LISLIEKENM EIEERERAEK KKRATKTPMV KFSAFS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SMARCA1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 1 (SMARCA1))

Alternative Name: Smarca1 (SMARCA1 Products)

Background: Probable global transcription activator SNF2L1 (EC 3.6.4.-) (ATP-dependent helicase SMARCA1) (DNA-dependent ATPase SNF2L) (Nucleosome-remodeling factor subunit SNF2L) (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1),FUNCTION: Helicase that possesses intrinsic ATP-dependent chromatin-remodeling activity (By similarity). ATPase activity is substrate-dependent, and is increased when nucleosomes are the substrate, but is also catalytically active when DNA alone is the substrate (By similarity). Catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (By similarity). Within the ISWI chromatin-remodeling complexes, slides edge- and center-positioned histone octamers away from their original location on the DNA template (By similarity). Catalytic activity and histone octamer sliding propensity is regulated and determined by components of the ISWI chromatin-remodeling complexes (By similarity). The BAZ1A-, BAZ1B-, BAZ2A- and BAZ2B-containing ISWI chromatin-remodeling complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template (By similarity). The CECR2- and RSF1-containing ISWI chromatin-remodeling complexes do not have the ability to slide mononucleosomes to the center of a DNA template (By similarity). Within the NURF-1 and CERF-1 ISWI chromatin remodeling complexes, nucleosomes are the preferred substrate for its ATPase activity (By similarity). Within the NURF-1 ISWI chromatin-remodeling complex, binds to the promoters of En1 and En2 to positively regulate their expression and promote brain development (By similarity). May promote neurite outgrowth (By similarity). May be involved in the development of luteal cells (By similarity). {ECO:0000250|UniProtKB:P28370}.

Molecular Weight: 121.7 kDa

UniProt: Q6PGB8

AlphaFold: Q6PGB8

Pathways: Chromatin Binding