DHX29 Protein (AA 1-1365) (His tag)

Catalog No. ABIN7564130

Product Details

Target: DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))

Protein Type: Recombinant

Protein Characteristics: AA 1-1365

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DHX29 protein is labelled with His tag.

Purpose: Custom-made recombinant Dhx29 Protein expressed in mammalian cells.

Sequence: MGGKNKKHKA PGAAAMRAAV SASRARSAEA GAVGEAQSKK PVARPAPAVP TGAREPRVKQ GPKIYSFNSA NDSGGSANLD KSILKVVINN KLEQRIIGVI NEHKKQNSDR GAISGRLSAK KLQDLYMALQ AFSFKTKDIE DAMTNTLLHG GDLHSALDWL CLNLSDDALP EGFSQEFEEQ QPKSRPKFQS VQIQATLSPP QQTKTKRQEE DPKIKPKKEE TTVEVNMKEW ILRYAEQQDE EEKGEGSKGL EEEEKFDPNQ RYLNLAARLL DAKEQAAAFK LEKNKQGQKE AQEKIRKFQR EMETLEDHPI FNPAIKISHQ QNEKKKPAPA TEAESALNLN LFEKSAAATE EEKGKKKEPH DVRNFDYTAR SWTGKSPKQF LIDWVRKNLP KSPNPSFEKV AVGRYWKCRV RVVRSEDDVL VVCPTILTED GMQAQHLGAT LALYRLVKGQ SVHQLLPPTY RDVWLEWSDE EKKREELNKM ETNKPRDLFI AKLLNKLKQQ QQQQQQQRPE SEKGGSEDPE ESWENLVSDE DLAALSLEPT SAEDLAPVRS LFRRLQSTPK YQRLLKERQQ LPVFKHRDSI VETLKRHRVV VVAGETGSGK STQVPHFLLE DLLLDECGAR KCNIVCTQPR RISAVSLATR VCEELGCESG PGGRNSLCGY QIRMESRASE STRLLYCTTG VLLRKLQEDG LLADVSHVIV DEVHERSVQS DFLLVILKEI LQKRSDLHLI LMSATVDSDK FSTYFTHCPI LRISGRSYPV EVFHLEDIVE ETGFVLEKDS EYCQKFLEEE EEITINVTSK AGGVKKYQEY IPVQSGASPE LNPFYQKYSS RTQHAILYMN PHKINLDLIL ELLVYLDKSP QFRNIEGAVL IFLPGLAHIQ QLYDLLSSDR RFYSERYQVI ALHSVLSTQD QAAAFMFPPP GVRKIVLATN IAETGITIPD VVFVIDTGRT KENKYHESSQ MSSLVETFVS KASALQRQGR AGRVRDGFCF RLYTRERFEG FLDYSVPEIL RVPLEELCLH IMKCDLGSPE DFLSKALDPP QLQVISNAMN LLRKIGACEP NEPKLTPLGQ HLAALPVNVK IGKMLIFGAI FGCLEPVATL AAVMTEKSPF ITPIGRKDEA DLAKSSLAVA DSDHLTIYNA YLGWKKAQQE GGFRSEISYC QRNFLNRTSL LTLEDVKQEL MKLVKAAGFS SSPSWEGRKG PQTLSFQDIA LLKAVLAAGL YDSVGKIMCT KSVDVTEKLA CMVETAQGKA QVHPSSVNRD LQTYGWLLYQ EKVRYTRVYL RETTLITPFP VLLFGGDIEV QHRERLLSVD GWIYFQAPVK IAVIFKQLRV LIDSVLRKKL ENPKMSLEND KILQIITELI KTENN Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))

Alternative Name: Dhx29 (DHX29 Products)

Background: ATP-dependent RNA helicase DHX29 (EC 3.6.4.13) (DEAH box protein 29),FUNCTION: ATP-binding RNA helicase involved in translation initiation. Part of the 43S pre-initiation complex that is required for efficient initiation on mRNAs of higher eukaryotes with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity. {ECO:0000255|HAMAP-Rule:MF_03068}.

Molecular Weight: 154.0 kDa

UniProt: Q6PGC1

AlphaFold: Q6PGC1