PIF1 Protein (AA 1-650) (His tag)

Catalog No. ABIN7564224

Product Details

Target: PIF1 (PIF1 5'-To-3' DNA Helicase (PIF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-650

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This PIF1 protein is labelled with His tag.

Purpose: Made-to-order recombinant Pif1 Protein expressed in mammalian cells.

Sequence: MRSGLCTPAE ALEMPSSTEA ATDECDDAEL RCRVAVEELS PGGQPRKRQA LRAAELSLGR NERRELMLRL QAPGPTGRPR CFPLRAVRLF TRFAATGRST LRLPTDGVPG AGSVQLLLSD CPPERLRRFL RTLRLKLAVA PGPGPASARA QLLGPRPRDF VTISPVQPEE LQRAAATKAP DSALEKRPME SQTSTEAPRW PLPVKKLRMP STKPKLSEEQ AAVLRMVLKG QSIFFTGSAG TGKSYLLKHI LGSLPPTGTV ATASTGVAAC HIGGTTLHAF AGIGSGQAPL AQCMALANRP GVRQGWLNCQ RLVIDEISMV EADFFDKLEA VARAVRQQKK PFGGIQLIIC GDFLQLPPVT KGSQQPQFCF QAKSWRRCVP VILELTEVWR QADQTFISLL QAVRLGRCSD EVTRQLRATA AHKVGRDGIV ATRLCTHQDD VALTNEKWLK ALPGDVHSFE AIDSDPELSR TLDAQCPVSR VLQLKLGAQV MLVKNLAVSR GLVNGARGVV VGFESEGRGL PRVRFLCGIT EVIRTDRWTV QVTGGQYLSR QQLPLQLAWA ISIHKSQGMS LDCVEISLGR VFASGQAYVA LSRARSLQGL RVLDFDPTVV RCDSRVLHFY ATLRQGRGLS LESQDDEEAN SDLENMDPNL Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PIF1 (PIF1 5'-To-3' DNA Helicase (PIF1))

Alternative Name: Pif1 (PIF1 Products)

Background: ATP-dependent DNA helicase PIF1 (EC 3.6.4.12) (DNA repair and recombination helicase PIF1) (Pif1/Rrm3 DNA helicase-like protein),FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity. {ECO:0000255|HAMAP-Rule:MF_03176}.

Molecular Weight: 70.9 kDa

UniProt: Q80SX8

AlphaFold: Q80SX8