DCAF5 Protein (AA 1-946) (His tag)

Catalog No. ABIN7564226

Product Details

Target: DCAF5 (DDB1 and CUL4 Associated Factor 5 (DCAF5))

Protein Type: Recombinant

Protein Characteristics: AA 1-946

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DCAF5 protein is labelled with His tag.

Purpose: Made-to-order recombinant Dcaf5 Protein expressed in mammalian cells.

Sequence: MKRRAGLGGS MRSVVGFLSQ RGLHGDPLLT QDFQRRRLRG CRNLYKKDLL GHFGCVNAIE FSNNGGQWLV SGGDDRRVLL WHMEQAIHSR VKPIQLKGEH HSNIFCLAFN SGNTKVFSGG NDEQVILHDV ESSETLDVFA HEDAVYGLSV SPVNDNIFAS SSDDGRVLIW DIRESPHGEP FCLANYPSAF HSVMFNPVEP RLLATANSKE GVGLWDIRKP QSSLLRYGGN LSLQSAMSVR FNSNGTQLLA LRRRLPPVLY DIHSRLPVFQ FDNQGYFNSC TMKSCCFAGD RDQYILSGSD DFNLYMWKIP ADPEAGGIGR VVNGAFMVLK GHRSIVNQVR FNPHTYMICS SGVEKIIKIW SPYKQPGCTG DLDGRIEDDS RCLYTHEEYI SLVLNSGSGL SHDYANQSVQ EDPRMMAFFD SLVRREIEGW SSDSDSDLSE STILQLHAGV SERSGYTDSE SSASLPRSPP PTVDESADNA FHLGPLRVTT TNAVASTPAT PTCEDASSRQ QRLSALRRYQ DKRLLALSNE SDSEENVCEA ELDTDLFPRP RSPSPEDGSS SPSSSTSSED EEELNQRRAT TRQRNAMRRR QKTARDERPT GPAKSTNTYI GEDNYDYPQI KVDDLSPSPD SSPERSASTL DVQPSRAPPA ANMESVERKI YKAYKWLRCS YISYSNNKDG ETSLMAEEAD EGRAGTSHKD NPTPSSSKEA CLTATAQRDQ DLPSEGCSSD ACKEGTSAGN PNSGAGHEHS SHPWAEVPEG TSQDTNNSGP LEHSFETKKL NGKALSKALS SRAEEPPASP GPKASGSTLN SASGNCPRTQ SDDSEERSLD TVCANHNNGR LHPRPLHPHN NGQSSGELET VACSSPGHLD TDHDSPSLTG TFLHKDCCGS EMACETPSAG MREDPPDPSG LDSSKVVHGQ SGLKRHRIEL EDTESENSSS EKKLKT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DCAF5 (DDB1 and CUL4 Associated Factor 5 (DCAF5))

Alternative Name: Dcaf5 (DCAF5 Products)

Background: DDB1- and CUL4-associated factor 5 (WD repeat-containing protein 22),FUNCTION: Is a substrate receptor for the CUL4-DDB1 E3 ubiquitin-protein ligase complex (CRL4), involved in the ubiquitination of a set of methylated non-histone proteins, including SOX2 (PubMed:30442713). The complex CRL4-DCAF5 is also involved in the ubiquitination of methylated DNMT1 and E2F1 (By similarity). {ECO:0000250|UniProtKB:Q96JK2, ECO:0000269|PubMed:30442713}.

Molecular Weight: 103.7 kDa

UniProt: Q80T85

AlphaFold: Q80T85