PPM1E Protein (AA 1-749) (His tag)
custom-made
PPM1E
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all PPM1E Proteins
- PPM1E (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1E (PPM1E))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-749
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PPM1E protein is labelled with His tag.
- Purpose
- Made-to-order recombinant Ppm1e Protein expressed in mammalian cells.
- Sequence
- MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPESEPE PEPEAELVAA EAAEASGEEP GEDAATVEAT EEGEQDQDPE PEDEAVEEET ATEGEEEEEE EAAAPGHSAV PPPPQPQLPP LPPLPRPLSE RITREEVEGE SLDLCLQQLY KYNCPSFLAA ALARATSDEV LQSDLSAHCI PKETDGTEGT VEIETVKLAR SVFSKLHEIC CSWVKDFPLR RRPQIYYETS IHAIKNMRRK MEDKHVCIPD FNMLFNLEDQ EEQAYFAVFD GHGGVDAAIY ASVHLHVNLV RQEMFPHDPA EALCRAFRVT DERFVQKAAR ESLRCGTTGV VTFIRGNMLH VAWVGDSQVM LVRKGQAVEL MKPHKPDRED EKQRIEALGG CVVWFGAWRV NGSLSVSRAI GDAEHKPYIC GDADSASTVL DGTEDYLILA CDGFYDTVNP DEAVKVVSDH LKENNGDSSM VAHKLVASAR DAGSSDNITV IVVFLRDMNK AVNVSEESEW TENSFQGGQE DGGDDKETHG ECKRPWPQHQ CSAPADLGYE GRVDSFTDRT SLSPGPQINV LEDPDYLDLT QIEASKPHST QFLPPVEMIG PGAPKKDLNE LIMEERSVKS SLPERSGAGE PRVSFNLGST GQQICRMENL SPVSSGLENE QFKSRGKTAS RLYHLRHHYS KRQRGFRFNP KFYSFLSARE PSHKIGISLS SLTRSGKRNK MLRSSLPWRE NSWEGYSGNV KIRKRNDIPC PDFPWSYKI Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- PPM1E (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1E (PPM1E))
- Alternative Name
- Ppm1e (PPM1E Products)
- Background
- Protein phosphatase 1E (EC 3.1.3.16) (Ca(2+)/calmodulin-dependent protein kinase phosphatase N) (CaMKP-N) (CaMKP-nucleus) (CaMKN) (Partner of PIX 1) (Partner of PIX-alpha) (Partner of PIXA),FUNCTION: Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42 (By similarity). Dephosphorylates PRKAA2. {ECO:0000250, ECO:0000269|PubMed:23088624}.
- Molecular Weight
- 83.4 kDa
- UniProt
- Q80TL0
- AlphaFold
- Q80TL0
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