LRWD1 Protein (AA 1-648) (His tag)

Catalog No. ABIN7564433

Product Details

Target: LRWD1 (Leucine-Rich Repeat and WD Repeat-Containing Protein 1 (LRWD1))

Protein Type: Recombinant

Protein Characteristics: AA 1-648

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This LRWD1 protein is labelled with His tag.

Purpose: Custom-made recombinant LRWD1 Protein expressed in mammalian cells.

Sequence: MAPLTPQLLL QRGRPKTDKL GKIQSLNLSG LQLLSEHLDP NLLGRLKKLR ELDLSNNLLE TLPANLGLSH LRILRCTNNQ LGDVTALHQF PELEELNLEG NPFLTVSDNL KVSFLLPKLR KVNGKDTAST CSQVENLDRE LMDRVTAHWQ KFIATVSPEE ETDKVRADFM RSAVRDVCYG PESLIEFTQW RVRMIAEELV ASGGAQVQDA KVPVEHPQAA GASKFRAREV ASKRPGKDPV TLPPSKRVRA LPPAQAEGSP MGADGGQAAL HLEPLHFLQC HSRNNSPKDL ETQLWACAFE PAREEGHSRA TSQTVATCGG EAVCVIDCQT GLVLHKYKVP GEEFFSVAWT ALTVVTQAGH KKRWNMLAAA GLRGMVRLLH VRAGFCCSVI RAHKKAIATL CFSPSHETHL FTASYDKRII LWDIGVPNQD YKFQASQLLT LNCGSVPLRL CPVATCPDDF LLAGCEGGCY CWDVRLDQPQ KQRVCEVNFI FSEDSKVSGQ RVDGLAFVNE DVVASKGSGQ GTIYLWSWSQ TWAGRGRQSV LPVVILVRLQ WSPTNLAYFS LSTCPGKNLV LCGDEEGSVW IYDVEHLLKE PPQATTLQPP TQILKWPQPT ALGQPVTKTM INTVVANAAF TYLTALTDSN IVSIWRRC Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: LRWD1 (Leucine-Rich Repeat and WD Repeat-Containing Protein 1 (LRWD1))

Alternative Name: LRWD1 (LRWD1 Products)

Background: Leucine-rich repeat and WD repeat-containing protein 1 (ORC-associated protein) (ORCA) (Origin recognition complex-associated protein),FUNCTION: Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation (By similarity). Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability. {ECO:0000250, ECO:0000269|PubMed:22427655}.

Molecular Weight: 71.6 kDa

UniProt: Q8BUI3

AlphaFold: Q8BUI3

Pathways: Chromatin Binding