PRMT7 Protein (AA 1-692) (His tag)
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- Target See all PRMT7 Proteins
- PRMT7 (Protein Arginine Methyltransferase 7 (PRMT7))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-692
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PRMT7 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Prmt7 Protein expressed in mammalian cells.
- Sequence
- MKVFCGRANP TTGSLEWLEE DEHYDYHQEI ARSSYADMLH DKDRNIKYYQ GIRAAVSRVK DRGQKALVLD IGTGTGLLSM MAVTAGADFC YAIEVFKPMA EAAVKIVERN GFSDKIKVIN KHSTEVTVGP DGDLPCRANI LITELFDTEL IGEGALPSYE HAHKHLVQED CEAVPHRATV YAQLVESRRM WSWNKLFPVR VRTSLGEQVI VPPSELERCP GAPSVCDIQL NQVSPADFTV LSDVLPMFSV DFSKQVSSSA ACHSRQFVPL ASGQAQVVLS WWDIEMDPEG KIKCTMAPFW AQTDPQELQW RDHWMQCVYF LPQEEPVVQG SPRCLVAHHD DYCVWYSLQR TSPDENDSAY QVRPVCDCQA HLLWNRPRFG EINDQDRTDH YAQALRTVLL PGSVCLCVSD GSLLSMLAHH LGAEQVFTVE SSVASYRLMK RIFKVNHLED KISVINKRPE LLTAADLEGK KVSLLLGEPF FTTSLLPWHN LYFWYVRTSV DQHLAPGAVV MPQAASLHAV IVEFRDLWRI RSPCGDCEGF DVHIMDDMIK HSLDFRESRE AEPHPLWEYP CRSLSKPQEI LTFDFQQPIP QQPMQSKGTM ELTRPGKSHG AVLWMEYQLT PDSTISTGLI NPAEDKGDCC WNPHCKQAVY FLSTTLDLRV PLNGPRSVSY VVEFHPLTGD ITMEFRLADT LS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Protein Arginine Methyltransferase 7 (PRMT7) (AA 1-692) protein (GST tag)
PRMT7 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Protein Arginine Methyltransferase 7 (PRMT7) (AA 1-692) protein (His tag)custom-made PRMT7 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Arginine Methyltransferase 7 (PRMT7) protein (DYKDDDDK Tag)PRMT7 Origin: Human Host: Baculovirus Recombinant EAA, ScA
Protein Arginine Methyltransferase 7 (PRMT7) protein (Myc-DYKDDDDK Tag)PRMT7 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- PRMT7 (Protein Arginine Methyltransferase 7 (PRMT7))
- Alternative Name
- Prmt7 (PRMT7 Products)
- Background
- Protein arginine N-methyltransferase 7 (EC 2.1.1.321) (Histone-arginine N-methyltransferase PRMT7) ([Myelin basic protein]-arginine N-methyltransferase PRMT7),FUNCTION: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro, the relevance of such results is however unclear in vivo (By similarity). {ECO:0000250, ECO:0000269|PubMed:17048991}.
- Molecular Weight
- 78.3 kDa
- UniProt
- Q922X9
- AlphaFold
- Q922X9
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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