RNF34 Protein (AA 1-376) (His tag)

Catalog No. ABIN7564889

Product Details

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Protein Type: Recombinant

Protein Characteristics: AA 1-376

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This RNF34 protein is labelled with His tag.

Purpose: Custom-made recombinant Rnf34 Protein expressed in mammalian cells.

Sequence: MKAGATSMWA SCCGLLNEVM GTGAVRGQQA GFPGSTGPFR FTPSSDFPTY PPAATEGPNI VCKACGLSFS VFRKKHVCCD CKKDFCSLCS VSQENLRRCS TCHLLQETAF QRPQLMRLKV KDLRQYLLLR NIPTDTCREK EDLVDLVLCH RGLGSGDDLD SSSLNSSRSQ TSSFFTQSLF SNYTPPSATV SSFQGELMDR DGAFRSEVLA QVQSEIASAN TDDDDDDDDD DDDDEDDDDE QEEEEQNPGL SKKKARASLS DLSSLEEVEG MSVRQLKEIL ARNFVNYSGC CEKWELVEKV NRLYKENEEN QKSYGERMQL QDEEDDSLCR ICMDAVIDCV LLECGHMVTC TKCGKRMSEC PICRQYVVRA VHVFKS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Alternative Name: Rnf34 (RNF34 Products)

Background: E3 ubiquitin-protein ligase RNF34 (EC 2.3.2.27) (Phafin-1) (RING finger protein 34) (RING-type E3 ubiquitin transferase RNF34),FUNCTION: E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation. {ECO:0000250|UniProtKB:Q969K3, ECO:0000269|PubMed:22064484}.

Molecular Weight: 42.0 kDa

UniProt: Q99KR6

AlphaFold: Q99KR6