CHAF1A Protein (AA 1-911) (His tag)
custom-made
CHAF1A
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all CHAF1A Proteins
- CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-911
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CHAF1A protein is labelled with His tag.
- Purpose
- Made-to-order recombinant Chaf1a Protein expressed in mammalian cells.
- Sequence
- MLEEPEAATR TAAAVDCKDR PGFPVKRLIQ ARLPFKRLNL VPKEKVEEDT SPKAAVESKV PDLQLSLGTF ESQCHTGSHV GLSTKLVGGQ GPIDSFLRAT IKPVPSVVII DLTENCSDIP DSPEGHSELS PDTAGVVTTV EGAAKQQEHS AAELCLLETP SDITCHMEEE PGSPGDPKRT GDCQAGSLQS CPELTPGSRT CPTKELSSWS KAGDLLFIEK VPVVVLEDIL ATKPSIASLP MMSLDRSVTS ESEILESCPE DDSILSHSST NSSSPTSSPE GPSTPPEHRG GRSSPSTPAC RVAKNFVKGS TEKGRSKLHR DREQQREEKE KLREEIRRAK EEARKKKEEE KELKEKERRE KREKDEKEKA EKQRLKEEKR KERQEALEAK LEEKRKKEEE KRLREEEKRL REEEKRIKAE KAEITRFFQK PKTPQAPKTL AGSCGKFAPF EIKEHMVLAP RCRAALDQDL CDQLDQLLQQ QSVASTFLSD LKSRLPLRSG PTRVCGHDTD IMNRDVVIVE SSKVDGVSER KKFGRMKLLQ FSENHRPAYW GTWNKKTAII RPRNPWAQDK DLLDYEVDSD DEWEEEEPGE SLSHSEGDED DDVGEDEDED DGFFVPHGYL SEDEGVTEEC ADPENHKVHQ KLKAKEWDEL LAKGKRFRVL QPVHVGCVWA SEAANCTSSD LKLLQQFTAC LLDVASPDEP EPGASRREKR DQHILAQLLP LLHGNVNGSK VIIHEFQEQC RRGLLTLPSP TPHLQMPNLE DAVAVPSKAR LKRLISENSA YEKRPNFRMC WYVHPEVLKS FGQECLPVPC QWTYITTMPS APREDSGSAS TEGPGQSTPM LLKRKPAATM CITQFMKKRR YDGQVGSGDM DGFQADTEED EEDDTDCMII DVPDVGSDVS EAPIPAPTLC K Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product CHAF1A Protein
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Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A) (AA 1-956) protein (His tag)
custom-made CHAF1A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A) protein (Myc-DYKDDDDK Tag)CHAF1A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))
- Alternative Name
- Chaf1a (CHAF1A Products)
- Background
- Chromatin assembly factor 1 subunit A (CAF-1 subunit A) (Chromatin assembly factor I p150 subunit) (CAF-I 150 kDa subunit) (CAF-I p150),FUNCTION: Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA, histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. {ECO:0000250|UniProtKB:Q5R1T0}.
- Molecular Weight
- 101.9 kDa
- UniProt
- Q9QWF0
- AlphaFold
- Q9QWF0
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