RFWD2 Protein (AA 1-733) (His tag)

Catalog No. ABIN7565184

Product Details

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Protein Type: Recombinant

Protein Characteristics: AA 1-733

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This RFWD2 protein is labelled with His tag.

Purpose: Custom-made recombinant Cop1 Protein expressed in mammalian cells.

Sequence: MSGSRQAGSG SAGTSPGSSA ASSVTSASSS LSSSPSPPSV AASAATLVSG GVAPAAGSGG LGGPGRPVLV AAAVSGSASA GGAVSAGQSR LSCAARPSAG VGGSSSSLGS SSRKRPLLVP LCNGLLNSYE DKSNDFVCPI CFDMIEEAYM TKCGHSFCYK CIHQSLEDNN RCPKCNYVVD NIDHLYPNFL VNELILKQKQ RFEEKRFKLD HSVSSTNGHR WQIFQDLLGT DQDNLDLANV NLMLELLVQK KKQLEAESHA AQLQILMEFL KVARRNKREQ LEQIQKELSV LEEDIKRVEE MSGLYSPVSE DSTVPQFEAP SPSHSSIIDS TEYSQPPGFS GTSQTKKQPW YNSTLASRRK RLTAHFEDLE QCYFSTRMSR ISDDSRTASQ LDEFQECLSK FTRYNSVRPL ATLSYASDLY NGSSIVSSIE FDRDCDYFAI AGVTKKIKVY EYGTVIQDAV DIHYPENEMT CNSKISCISW SSYHKNLLAS SDYEGTVILW DGFTGQRSKV YQEHEKRCWS VDFNLMDPKL LASGSDDAKV KLWSTNLDNS VASIEAKANV CCVKFSPSSR YHLAFGCADH CVHYYDLRNT KQPIMVFKGH RKAVSYAKFV SGEEIVSAST DSQLKLWNVG KPYCLRSFKG HINEKNFVGL ASNGDYIACG SENNSLYLYY KGLSKTLLTF KFDTVKSVLD KDRKEDDTNE FVSAVCWRAL SDGESNVLIA ANSQGTIKVL ELV Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))

Alternative Name: Cop1 (RFWD2 Products)

Background: E3 ubiquitin-protein ligase COP1 (EC 2.3.2.27) (Constitutive photomorphogenesis protein 1 homolog) (mCOP1) (RING finger and WD repeat domain protein 2) (RING-type E3 ubiquitin transferase RFWD2),FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif. {ECO:0000250|UniProtKB:Q8NHY2}.

Molecular Weight: 80.4 kDa

UniProt: Q9R1A8

AlphaFold: Q9R1A8

Pathways: Photoperiodism